Simitzi Chara, Vlahovic Maja, Georgiou Alex, Keskin-Erdogan Zalike, Miller Joanna, Day Richard M
Centre for Precision Healthcare, Applied Biomedical Engineering Group, UCL Division of Medicine, University College London, London, United Kingdom.
Department of Biomolecular and Sports Sciences, Coventry University, Coventry, United Kingdom.
Front Bioeng Biotechnol. 2020 Jul 22;8:816. doi: 10.3389/fbioe.2020.00816. eCollection 2020.
Mesenchymal stromal cells (MSC) hold significant potential for tissue engineering applications. Modular tissue engineering involves the use of cellularized "building blocks" that can be assembled via a bottom-up approach into larger tissue-like constructs. This approach emulates more closely the complexity associated hierarchical tissues compared with conventional top-down tissue engineering strategies. The current study describes the combination of biodegradable porous poly(DL-lactide-co-glycolide) (PLGA) TIPS microcarriers with canine adipose-derived MSC (cAdMSC) for use as implantable conformable building blocks in modular tissue engineering applications. Optimal conditions were identified for the attachment and proliferation of cAdMSC on the surface of the microcarriers. Culture of the cellularized microcarriers for 21 days in transwell insert plates under conditions used to induce either chondrogenic or osteogenic differentiation resulted in self-assembly of solid 3D tissue constructs. The tissue constructs exhibited phenotypic characteristics indicative of successful osteogenic or chondrogenic differentiation, as well as viscoelastic mechanical properties. This strategy paves the way to create tissue engineered constructs via modular tissue engineering for therapeutic applications.
间充质基质细胞（MSC）在组织工程应用中具有巨大潜力。模块化组织工程涉及使用细胞化的“构建模块”，这些模块可以通过自下而上的方法组装成更大的组织样构建体。与传统的自上而下的组织工程策略相比，这种方法更紧密地模拟了与分层组织相关的复杂性。当前的研究描述了将可生物降解的多孔聚（DL-丙交酯-共-乙交酯）（PLGA）热致相分离微载体与犬脂肪来源的MSC（cAdMSC）相结合，用作模块化组织工程应用中可植入的顺应性构建模块。确定了cAdMSC在微载体表面附着和增殖的最佳条件。在用于诱导软骨生成或成骨分化的条件下，将细胞化的微载体在transwell插入板中培养21天，导致形成固体三维组织构建体的自组装。这些组织构建体表现出表明成功进行成骨或软骨生成分化的表型特征以及粘弹性力学性能。该策略为通过模块化组织工程创建用于治疗应用的组织工程构建体铺平了道路。
