Berry David B, Englund Erin K, Chen Shaochen, Frank Lawrence R, Ward Samuel R
Departments of NanoEngineering, University of California, San Diego, USA.
Biomater Sci. 2021 Jan 21;9(2):301-314. doi: 10.1039/d0bm00705f. Epub 2020 Aug 10.
Advancement of tissue engineering and regenerative medicine (TERM) strategies to replicate tissue structure and function has led to the need for noninvasive assessment of key outcome measures of a construct's state, biocompatibility, and function. Histology based approaches are traditionally used in pre-clinical animal experiments, but are not always feasible or practical if a TERM construct is going to be tested for human use. In order to transition these therapies from benchtop to bedside, rigorously validated imaging techniques must be utilized that are sensitive to key outcome measures that fulfill the FDA standards for TERM construct evaluation. This review discusses key outcome measures for TERM constructs and various clinical- and research-based imaging techniques that can be used to assess them. Potential applications and limitations of these techniques are discussed, as well as resources for the processing, analysis, and interpretation of biomedical images.
组织工程与再生医学（TERM）策略在复制组织结构和功能方面的进展，导致需要对构建体的状态、生物相容性和功能的关键结果指标进行无创评估。基于组织学的方法传统上用于临床前动物实验，但如果要对TERM构建体进行人体使用测试，并不总是可行或实际的。为了将这些疗法从实验室过渡到临床，必须采用经过严格验证的成像技术，这些技术对符合FDA TERM构建体评估标准的关键结果指标敏感。本综述讨论了TERM构建体的关键结果指标以及可用于评估它们的各种基于临床和研究的成像技术。讨论了这些技术的潜在应用和局限性，以及生物医学图像的处理、分析和解释资源。
