College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Anim Biotechnol. 2022 Jun;33(3):448-456. doi: 10.1080/10495398.2020.1803343. Epub 2020 Aug 10.
Meat quality is closely related to the fat deposition which is regulated by a cascade of transcription factors. As a transcription factor, the CCAAT/enhancer binding protein alpha (CEBPA) is considered as one of the key molecules regulating adipogenesis. Therefore, the objective of this study was to detect the expression pattern of the gene and evaluate whether its single nucleotide polymorphisms (SNPs) were associated with the meat quality traits in Wuliang Mountain Black-bone (WLMB) chickens. The results showed that the chicken mRNA was widely expressed in the 11 tissues, and the expression pattern of it might be tissue- and time-specific different. The locus of g.74C > G was not significantly associated with chicken meat quality. For the locus of g.552G > A, chickens with the GG genotype showed higher pH ( < 0.01), lower drip loss ( < 0.01) and higher intramuscular fat ( < 0.05) than those with other genotypes. It suggested that polymorphisms of the gene were significantly associated with the meat quality traits of WLMB chickens. The results of this study contribute to the functional research of the gene and lay the foundation for improving meat quality based on the marker-assisted selection in chickens.
肉品质与脂肪沉积密切相关，脂肪沉积受一系列转录因子的调控。作为转录因子，CCAAT/增强子结合蛋白α（CEBPA）被认为是调节脂肪生成的关键分子之一。因此，本研究旨在检测基因的表达模式，并评估其单核苷酸多态性（SNPs）是否与乌蒙山黑骨鸡（WLMB）的肉质性状相关。结果表明，鸡基因在 11 种组织中广泛表达，其表达模式可能具有组织和时间特异性。g.74C>G 位点与鸡肉质性状无显著相关性。对于 g.552G>A 位点，GG 基因型的鸡表现出更高的 pH 值（<0.01）、更低的滴水损失（<0.01）和更高的肌内脂肪（<0.05），而其他基因型的鸡则较低。这表明基因的多态性与 WLMB 鸡的肉质性状显著相关。本研究的结果为该基因的功能研究提供了依据，并为基于标记辅助选择改善鸡的肉质奠定了基础。
