Department of Psychiatry, Columbia University, 1051 Riverside Drive, New York, NY 10032, USA.
Department of Neuroscience, New York State Psychiatric Institute, 1051 Riverside Drive, New York, NY 10032, USA.
Biotechniques. 2020 Oct;69(4):307-312. doi: 10.2144/btn-2020-0050. Epub 2020 Aug 11.
The increasing interest in manipulating neural circuits in developing brains has created a demand for reliable and accurate methods for delivering viruses to newborn mice. Here we describe a novel 3D-printed mouse neonatal stereotaxic adaptor for intracerebral viral injection that provides enhanced precision and reliability. Using this device, we injected mice with a -dependent hM4D-mCherry viral construct at postnatal day 1 (P1) and demonstrated selective expression in the striatal indirect pathway neurons on days P7, P11 and P25. Similarly, dopaminergic midbrain neurons were selectively targeted with a -dependent green fluorescent protein virus in -IRES- neonates and expression examined at P25. Our open-source neonatal stereotaxic mouse adaptor facilitates neonatal neuronal targeting, which should improve the ability to label and modify neural circuits in developing mouse brains.
人们对在发育中的大脑中操纵神经回路越来越感兴趣，这就需要可靠和精确的方法将病毒递送到新生小鼠体内。在这里，我们描述了一种新颖的 3D 打印小鼠新生立体定位适配器，用于脑内病毒注射，可提高精度和可靠性。使用该设备，我们在出生后第 1 天（P1）向 小鼠注射了依赖于 的 hM4D-mCherry 病毒构建体，并在 P7、P11 和 P25 日观察到纹状体间接通路神经元的选择性表达。同样，依赖于 的绿色荧光蛋白病毒在 -IRES- 新生儿中也可选择性靶向多巴胺能中脑神经元，并在 P25 时检测到表达。我们的开源新生儿立体定位小鼠适配器促进了新生神经元的靶向，这应该会提高在发育中的小鼠大脑中标记和修饰神经回路的能力。
