Dubnau E, Weir J, Nair G, Carter L, Moran C, Smith I
Department of Microbiology, Public Health Research Institute, New York, New York 10016.
J Bacteriol. 1988 Mar;170(3):1054-62. doi: 10.1128/jb.170.3.1054-1062.1988.
The DNA sequences of the spo0H genes from Bacillus licheniformis and B. subtilis are described, and the predicted open reading frames code for proteins of 26,097 and 25,447 daltons, respectively. The two spo0H gene products are 91% identical to one another and about 25% identical to most of the procaryotic sigma factors. The predicted proteins have a conserved 14-amino-acid sequence at their amino terminal end, typical of sigma factors. Antibodies raised against the spo0H gene product of B. licheniformis specifically react with RNA polymerase sigma factor protein, sigma 30, purified from B. subtilis. We conclude that the spo0H genes of B. licheniformis and B. subtilis code for sigma 30, now known as sigma H.
本文描述了地衣芽孢杆菌和枯草芽孢杆菌spo0H基因的DNA序列，预测的开放阅读框分别编码26,097和25,447道尔顿的蛋白质。这两种spo0H基因产物彼此间有91%的同源性，与大多数原核σ因子约有25%的同源性。预测的蛋白质在其氨基末端有一个保守的14个氨基酸序列，这是σ因子的典型特征。针对地衣芽孢杆菌spo0H基因产物产生的抗体与从枯草芽孢杆菌中纯化的RNA聚合酶σ因子蛋白σ30发生特异性反应。我们得出结论，地衣芽孢杆菌和枯草芽孢杆菌的spo0H基因编码σ30，即现在所知的σH。
