出血性结肠炎：临床微生物实验室中产生志贺毒素的大肠杆菌O157的检测
Haemorrhagic colitis: detection of verotoxin producing Escherichia coli O157 in a clinical microbiology laboratory.
作者信息
Walker C W, Upson R, Warren R E
机构信息
Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge.
出版信息
J Clin Pathol. 1988 Jan;41(1):80-4. doi: 10.1136/jcp.41.1.80.
Abstract
Faeces (n = 1319) were examined over three months for the presence of non-sorbitol fermenting, verotoxin producing Escherichia coli (serotype O157). Seven isolates were found, four from patients with bloodstained diarrhoea and three from patients with no evidence of blood in the faeces. Screening of all faecal samples with specific O157 antiserum for non-sorbitol fermenting organisms and agglutination was an important adjunct to clinical and microscopic findings and helped detect cases of verotoxin producing E coli which might otherwise have been missed.
摘要
在三个月的时间里，对1319份粪便样本进行了检测，以查找非山梨醇发酵、产志贺毒素大肠杆菌（血清型O157）。发现了7株分离菌，其中4株来自有便血腹泻的患者，3株来自粪便中无血液迹象的患者。用O157特异性抗血清对所有粪便样本进行非山梨醇发酵菌的筛查和凝集试验，是对临床和显微镜检查结果的重要辅助手段，有助于检测出可能漏诊的产志贺毒素大肠杆菌病例。
相似文献
1
Haemorrhagic colitis: detection of verotoxin producing Escherichia coli O157 in a clinical microbiology laboratory.出血性结肠炎：临床微生物实验室中产生志贺毒素的大肠杆菌O157的检测
J Clin Pathol. 1988 Jan;41(1):80-4. doi: 10.1136/jcp.41.1.80.
2
The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains of Escherichia coli O 157.使用山梨醇麦康凯琼脂并结合一种特定抗血清来检测产志贺毒素大肠杆菌O157菌株。
Epidemiol Infect. 1988 Oct;101(2):327-35. doi: 10.1017/s0950268800054261.
3
Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis.与出血性结肠炎相关的产志贺毒素大肠埃希菌
J Clin Microbiol. 1987 Aug;25(8):1486-9. doi: 10.1128/jcm.25.8.1486-1489.1987.
4
Diagnosis of infections with Shiga-like toxin-producing Escherichia coli by use of enzyme-linked immunosorbent assays for Shiga-like toxins on cultured stool samples.通过对培养的粪便样本进行志贺样毒素的酶联免疫吸附测定来诊断产志贺样毒素大肠杆菌感染。
J Med Microbiol. 1994 Apr;40(4):241-5. doi: 10.1099/00222615-40-4-241.
5
[Gastroenteritis caused by verotoxin-producing Escherichia coli O157. Presentation of 2 cases].[产志贺毒素大肠杆菌O157引起的肠胃炎。2例病例报告]
Enferm Infecc Microbiol Clin. 1992 Jun-Jul;10(6):349-51.
6
Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source.谢菲尔德产志贺毒素大肠杆菌感染：牛作为可能的传染源
Epidemiol Infect. 1989 Jun;102(3):439-45. doi: 10.1017/s0950268800030156.
7
Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7.由大肠杆菌O157:H7引起的出血性结肠炎暴发的实验室调查。
J Clin Microbiol. 1987 Jun;25(6):1043-7. doi: 10.1128/jcm.25.6.1043-1047.1987.
8
A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from cases of bloody diarrhoea, non-bloody diarrhoea and asymptomatic contacts.采用免疫磁珠分离法与直接培养法从血性腹泻、非血性腹泻病例及无症状接触者中分离产志贺毒素大肠杆菌O157的比较
J Med Microbiol. 1996 Apr;44(4):267-71. doi: 10.1099/00222615-44-4-267.
9
Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis.用于检测与出血性结肠炎相关的大肠杆菌O157:H7的山梨醇麦康凯培养基。
J Clin Microbiol. 1986 May;23(5):869-72. doi: 10.1128/jcm.23.5.869-872.1986.
10
Vero cytotoxin-producing strains of Escherichia coli.产志贺毒素大肠杆菌菌株
J Med Microbiol. 1988 Jun;26(2):77-85. doi: 10.1099/00222615-26-2-77.
引用本文的文献
1
The isolation rate of Escherichia coli 0157:H7 in Toronto and surrounding communities.多伦多及周边社区大肠杆菌O157:H7的分离率。
Can J Infect Dis. 1990 Spring;1(1):23-7. doi: 10.1155/1990/583209.
2
Prospective study of verocytotoxin-producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states.不同腹泻状态下产志贺毒素、肠集聚性和弥漫性黏附大肠杆菌的前瞻性研究。
Epidemiol Infect. 1994 Feb;112(1):63-7. doi: 10.1017/s0950268800057423.
3
Sorbitol-negative phenotype among enterohemorrhagic Escherichia coli strains of different serotypes and from different sources.不同血清型及不同来源的肠出血性大肠杆菌菌株中的山梨醇阴性表型。
J Clin Microbiol. 1995 Aug;33(8):2199-201. doi: 10.1128/jcm.33.8.2199-2201.1995.
4
Haemorrhagic colitis and Escherichia coli O157--a pathogen unmasked.出血性结肠炎与大肠杆菌O157——一种被揭示的病原体
Br Med J (Clin Res Ed). 1988 Mar 26;296(6626):875-6. doi: 10.1136/bmj.296.6626.875.
5
The use of sorbitol-MacConkey agar in conjunction with a specific antiserum for the detection of Vero cytotoxin-producing strains of Escherichia coli O 157.使用山梨醇麦康凯琼脂并结合一种特定抗血清来检测产志贺毒素大肠杆菌O157菌株。
Epidemiol Infect. 1988 Oct;101(2):327-35. doi: 10.1017/s0950268800054261.
6
Vero toxin producing E coli in haemorrhagic colitis.产志贺毒素大肠杆菌与出血性结肠炎
J Clin Pathol. 1988 Aug;41(8):923. doi: 10.1136/jcp.41.8.923-c.
7
Clinical and pathological variability of infection by enterohaemorrhagic (Vero cytotoxin producing) Escherichia coli.肠出血性（产志贺毒素）大肠杆菌感染的临床和病理变异性
J Clin Pathol. 1989 Aug;42(8):847-52. doi: 10.1136/jcp.42.8.847.
8
Results of screening for verocytotoxin-producing Escherichia coli in faeces in Belgium.比利时粪便中产志贺毒素大肠杆菌的筛查结果。
Eur J Clin Microbiol Infect Dis. 1990 Mar;9(3):198-201. doi: 10.1007/BF01963837.
本文引用的文献
1
Activity of lysine decarboxlase as an aid in the identification of Salmonellae and Shigellae.赖氨酸脱羧酶活性在沙门氏菌和志贺氏菌鉴定中的辅助作用。
Am J Clin Pathol. 1958 Jun;29(6):598-600. doi: 10.1093/ajcp/29.6_ts.598.
2
Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis.与出血性结肠炎相关的大肠杆菌的存活及生长特性
Appl Environ Microbiol. 1984 Oct;48(4):855-6. doi: 10.1128/aem.48.4.855-856.1984.
3
Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features.散发性与大肠杆菌O157:H7相关的出血性结肠炎病例。临床、流行病学和细菌学特征。
Ann Intern Med. 1984 Dec;101(6):738-42. doi: 10.7326/0003-4819-101-6-738.
4
Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype.与一种罕见的大肠杆菌血清型相关的出血性结肠炎疫情的实验室调查。
J Clin Microbiol. 1983 Sep;18(3):512-20. doi: 10.1128/jcm.18.3.512-520.1983.
5
Hemorrhagic colitis associated with a rare Escherichia coli serotype.与一种罕见大肠杆菌血清型相关的出血性结肠炎。
N Engl J Med. 1983 Mar 24;308(12):681-5. doi: 10.1056/NEJM198303243081203.
6
Escherichia coli O157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (SHIGA) like cytotoxin.在美国，与出血性结肠炎相关的大肠杆菌O157:H7菌株会产生一种志贺氏痢疾杆菌1型（志贺毒素）样细胞毒素。
Lancet. 1983 Mar 26;1(8326 Pt 1):702. doi: 10.1016/s0140-6736(83)91987-6.
7
Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools.散发性溶血尿毒综合征病例，与粪便中的细胞毒素及产细胞毒素大肠杆菌有关。
Lancet. 1983 Mar 19;1(8325):619-20. doi: 10.1016/s0140-6736(83)91795-6.
8
Cytotoxic Escherichia coli O157:H7 associated with haemorrhagic colitis in Canada.与加拿大出血性结肠炎相关的细胞毒性大肠杆菌O157:H7
Lancet. 1983 Jan 1;1(8314-5):76. doi: 10.1016/s0140-6736(83)91616-1.
9
H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis.H7抗血清-山梨醇发酵培养基：一种用于检测与出血性结肠炎相关的大肠杆菌O157:H7的单管筛选培养基。
J Clin Microbiol. 1985 Oct;22(4):620-5. doi: 10.1128/jcm.22.4.620-625.1985.
10
Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin.通过菌落扫描和多粘菌素提取志贺毒素检测混合培养物中少量产志贺毒素大肠杆菌的灵敏方法。
J Clin Microbiol. 1985 Oct;22(4):614-9. doi: 10.1128/jcm.22.4.614-619.1985.
Zotero 插件