Department of Urology, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, 310003, Zhejiang, China.
Mol Cell Biochem. 2020 Nov;474(1-2):83-94. doi: 10.1007/s11010-020-03835-2. Epub 2020 Aug 12.
The abnormal expression of circular RNA (circRNA) is bound up with the progress of various human cancers. This study aimed to reveal the potential role and mechanism of circBC048201 in the proliferation, migration, and invasion of bladder cancer cells. Quantitative real-time PCR was performed to detect the expression of circBC048201. Cell Counting Kit-8, colony formation, and transwell migration and invasion assays were used to confirm the in vitro functions of circBC048201. Western blot, RNA pull-down, and dual-luciferase reporter gene experiments were performed to study the potential mechanism. circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited bladder cancer cell proliferation, migration, and invasion. From the potential mechanism analysis, our data suggested that circBC048201 and miR-1184, miR-1184 and ITGA3 could bind to each other, and the interference with circBC048201 repressed bladder cancer cell proliferation, migration, and invasion through the miR-1184/ITGA3 axis. In summary, our results showed that circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited the proliferation, migration, and invasion of bladder cancer cells through the miR-1184/ITGA3 axis.
环状 RNA（circRNA）的异常表达与各种人类癌症的进展有关。本研究旨在揭示 circBC048201 在膀胱癌细胞增殖、迁移和侵袭中的潜在作用和机制。采用实时定量 PCR 检测 circBC048201 的表达。采用细胞计数试剂盒-8、集落形成、Transwell 迁移和侵袭实验证实 circBC048201 的体外功能。采用 Western blot、RNA 下拉和双荧光素酶报告基因实验研究潜在机制。circBC048201 在膀胱癌组织和细胞中异常高表达，干扰 circBC048201 抑制膀胱癌细胞增殖、迁移和侵袭。从潜在机制分析，我们的数据表明 circBC048201 与 miR-1184、miR-1184 与 ITGA3 可以相互结合，干扰 circBC048201 通过 miR-1184/ITGA3 轴抑制膀胱癌细胞增殖、迁移和侵袭。总之，我们的研究结果表明，circBC048201 在膀胱癌组织和细胞中异常高表达，干扰 circBC048201 通过 miR-1184/ITGA3 轴抑制膀胱癌细胞增殖、迁移和侵袭。
