Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Faculty of Medicine, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, Ceará, CEP: 60.416-030, Brazil.
Post Graduate Program in Pharmacology, Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Inflammation. 2021 Feb;44(1):116-128. doi: 10.1007/s10753-020-01313-x.
Periodontitis and rheumatoid arthritis (RA) are inflammatory diseases characterized by chronic inflammation and bone erosion. Electroacupuncture (EA) shows anti-inflammatory and anti-resorptive effects in experimental periodontitis (EP) and in RA. It is important to investigate whether EA shows these effects in periodontal tissues in the presence of these two inflammatory diseases or not. For this, Wistar rats were divided into six groups: control (C); experimental rheumatoid arthritis (RA; bovine type II collagen-induced (CII)); experimental periodontitis (EP); RA/EP (RA + EP); EP/EA (EP treated with EA); RA/EP/EA (RA + EP treated with EA). EP was induced 21 days after RA induction and EA was performed previously and during the EP induction period, every 3 days until the 36th experimental day. The rats were euthanized on day 39. RA was evaluated by edema and the withdrawal threshold of hind paws. The maxillae were removed, and alveolar bone loss (ABL) and bone radiographic density (BRD) were evaluated. Immunohistochemical analyses for interleukins (IL)-6 and -17 and nuclear factor (NF)-κB were performed. Our results showed that EA reduced only the pain intensity in arthritic rats. Histomorphometric, macroscopic, and radiographic analyses did not show differences between the control and EP/EA groups. EA caused a reduction in ABL and BRD only in the presence of EP. EA caused a reduction in IL-6 and -17 in all groups, but NF-κB was only reduced in the arthritic rats with EP. In conclusion, EA reduced the inflammation related to periodontitis in arthritic rats but did not prevent ABL.
牙周炎和类风湿关节炎（RA）是两种以慢性炎症和骨侵蚀为特征的炎症性疾病。电针（EA）在实验性牙周炎（EP）和 RA 中表现出抗炎和抗吸收作用。重要的是要研究在存在这两种炎症性疾病的情况下，EA 是否在牙周组织中表现出这些作用。为此，将 Wistar 大鼠分为六组：对照组（C）；实验性类风湿关节炎（RA；牛 II 型胶原诱导的（CII））；实验性牙周炎（EP）；RA/EP（RA + EP）；EP/EA（EP 用 EA 治疗）；RA/EP/EA（RA + EP 用 EA 治疗）。RA 在 EP 诱导后 21 天诱导，EA 在 EP 诱导期间之前和期间每 3 天进行一次，直到第 36 个实验日。第 39 天处死大鼠。通过后爪撤回阈值评估 RA 的肿胀情况。取出上颌骨，评估牙槽骨丧失（ABL）和骨放射性密度（BRD）。进行白细胞介素（IL）-6 和 -17 和核因子（NF）-κB 的免疫组织化学分析。我们的结果表明，EA 仅减轻了关节炎大鼠的疼痛强度。组织形态计量学、宏观和放射照相分析显示，对照组和 EP/EA 组之间没有差异。仅在存在 EP 的情况下，EA 才会减少 ABL 和 BRD。EA 仅在存在 EP 的情况下降低了所有组的 IL-6 和 -17，但仅在关节炎大鼠中降低了 NF-κB。总之，EA 减轻了关节炎大鼠与牙周炎相关的炎症，但不能预防 ABL。
