电针通过抑制TLR2/4-MyD88-NF-κB信号通路减轻胶原诱导性关节炎大鼠的疼痛反应和炎症。

Electroacupuncture Alleviates Pain Responses and Inflammation in Collagen-Induced Arthritis Rats via Suppressing the TLR2/4-MyD88-NF-B Signaling Pathway.

作者信息

Sun Shi-Yue, Yan Qi-Qi, Qiao Li-Na, Shi Yi-Nan, Tan Lian-Hong, Yang Yong-Sheng

机构信息

Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Evid Based Complement Alternat Med. 2023 Feb 4;2023:9050763. doi: 10.1155/2023/9050763. eCollection 2023.

RESULTS

EA intervention and OxPAPC injection could relieve mechanical allodynia and thermal hyperalgesia caused by CIA. Paw edema and pathological damage of synovium were significantly ameliorated after EA intervention and OxPAPC injection. Furthermore, EA intervention and OxPAPC injection markedly reduced the contents of serum TNF-, IL-1, and IL-6, as well as the protein expression levels of synovial TLR2, TLR4, MyD88, and NF-B p-p65. In particular, the expression of TLR2 and TLR4 on synovial fibroblasts and macrophages in synovium was significantly reduced by EA intervention.

CONCLUSIONS

Repeated EA stimulation at ST36 and SP6 can effectively relieve joint pain and synovial inflammation caused by RA in CIA rats. The analgesic and anti-inflammatory effect of EA may be closely related to the inhibition of innate immune responses driven by the TLR2/4-MyD88-NF-B signaling pathway in the synovium.

结果

电针干预和氧化型磷脂酰乙醇胺（OxPAPC）注射可减轻胶原诱导性关节炎（CIA）所致的机械性异常性疼痛和热痛觉过敏。电针干预和OxPAPC注射后，爪部水肿和滑膜病理损伤明显改善。此外，电针干预和OxPAPC注射显著降低了血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）的含量，以及滑膜Toll样受体2（TLR2）、Toll样受体4（TLR4）、髓样分化因子88（MyD88）和核因子-κB p-p65的蛋白表达水平。特别是，电针干预显著降低了滑膜中成纤维细胞和巨噬细胞上TLR2和TLR4的表达。