Tojo M, Yamashita N, Goldmann D A, Pier G B
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
J Infect Dis. 1988 Apr;157(4):713-22. doi: 10.1093/infdis/157.4.713.
We isolated a polysaccharide adhesin from Staphylococcus epidermidis strain RP-62A. The adhesin was composed of a complex mix of monosaccharides (with galactose and glucosamine predominating), bound well to silastic catheter tubing, inhibited adherence of strain RP-62A to catheters, and elicited antibodies that both blocked adherence and stabilized an extracellular structure (visualized by transmission electron microscopy) that appeared to be a capsule. Two of three heterologous, highly adherent strains of coagulase-negative staphylococci also produced this adhesin, and their adherence to catheters was inhibited by both purified adhesin and antibody to adhesin. In contrast, the adherence of one highly adherent and two poorly adherent heterologous strains was unaffected by the RP-62A purified adhesin or antibody, a result suggesting the expression of alternate adhesins by these strains. We conclude that the capsular polysaccharide of some strains of coagulase-negative staphylococci is an important factor in adherence to catheter tubing.
我们从表皮葡萄球菌RP - 62A菌株中分离出一种多糖黏附素。该黏附素由单糖的复杂混合物组成（以半乳糖和氨基葡萄糖为主），能很好地结合到硅橡胶导管上，抑制RP - 62A菌株对导管的黏附，并引发抗体，这些抗体既能阻断黏附，又能稳定一种通过透射电子显微镜观察到的似乎是荚膜的细胞外结构。三株异源的、高度黏附的凝固酶阴性葡萄球菌中有两株也产生这种黏附素，它们对导管的黏附被纯化的黏附素和抗黏附素抗体所抑制。相比之下，一株高度黏附的和两株低黏附的异源菌株的黏附不受RP - 62A纯化黏附素或抗体的影响，这一结果表明这些菌株表达了其他黏附素。我们得出结论，某些凝固酶阴性葡萄球菌菌株的荚膜多糖是其黏附到导管上的一个重要因素。
