Kallscheuer Nicolai, Kumar Gaurav, Ahamad Shabbir, Duddeda Sandhya, Sasikala Chintalapati, Jogler Christian, Ramana Chintalapati Venkata
Department of Microbial Interactions, Institute of Microbiology, Friedrich Schiller University, 07743, Jena, Germany.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India.
Antonie Van Leeuwenhoek. 2025 Jul 11;118(8):111. doi: 10.1007/s10482-025-02106-8.
A pink-coloured, salt- and alkali-tolerant strain, JC639, was isolated from a specimen of the marine sponge Spheciospongia sp. The novel isolate is motile, aerobic and forms oval to pear-shaped cells. It shares the highest 16S rRNA gene sequence identity with Stieleria neptunia Enr13 and Stieleria maiorica Mal15 in the family Pirellulaceae (phylum Planctomycetota). Optimum pH and temperature for growth are 8.0 (range 7.0-10.0) and 25 °C (range 10-30 °C), respectively. Major fatty acids are Cω8c, C Cω9c, C, and Cω7c/Cω6c. Major polar lipids are phosphatidylcholine and phosphatidylethanolamine. The genome of strain JC639 has a size of 9.56 Mb and a DNA G + C content of 59.5%. The polyphasic analysis of the isolate supports its delineation from the known species in the genus Stieleria. We therefore introduce Stieleria mannarensis sp. nov., represented by JC639 (= KCTC 72168 = NBRC 113878) as the type strain. Based on an analysis of established phylogenomic markers performed with all current members of the overlapping genera Stieleria and Roseiconus, we further propose to include the two so far characterized Roseiconus species in the genus Stieleria.
从海洋海绵Spheciospongia sp.的一个标本中分离出一株耐盐和耐碱的粉红色菌株JC639。该新分离株具有运动性，为需氧型，形成椭圆形至梨形细胞。它与皮氏菌科（浮霉菌门）的海王星斯氏菌Enr13和马略卡岛斯氏菌Mal15的16S rRNA基因序列同一性最高。生长的最适pH值和温度分别为8.0（范围为7.0 - 10.0）和25℃（范围为10 - 30℃）。主要脂肪酸为Cω8c、Cω9c、C以及Cω7c/Cω6c。主要极性脂为磷脂酰胆碱和磷脂酰乙醇胺。菌株JC639的基因组大小为9.56 Mb，DNA G + C含量为59.5%。对该分离株的多相分析支持将其与斯氏菌属中的已知物种区分开来。因此，我们引入甘露那瑞斯氏菌新种，以JC639（= KCTC 72168 = NBRC 113878）作为模式菌株。基于对重叠属斯氏菌属和玫瑰锥菌属所有现有成员进行的既定系统发育基因组标记分析，我们进一步提议将迄今已鉴定的两个玫瑰锥菌属物种归入斯氏菌属。
