State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, PKU-Nanjing Institute of Translational Medicine, Peking University, Beijing, 100871, China.
Academy of Advanced Interdisciplinary Study, Peking University, Beijing, 100871, China.
Neurosci Bull. 2020 Oct;36(10):1182-1190. doi: 10.1007/s12264-020-00561-z. Epub 2020 Aug 14.
An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular, cellular, circuit, and system levels. The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors. In this brief review, we summarize recent advances in miniature fluorescence microscopy for neuroscience, focusing mostly on two mainstream solutions - miniature single-photon microscopy, and miniature two-photon microscopy. We discuss their technical advantages and limitations as well as unmet challenges for future improvement. Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements, long and complex protocols, and even disease progression and aging.
神经科学的终极目标是在分子、细胞、回路和系统水平上破译神经元信息处理的原理。微型荧光显微镜的出现通过可视化动物自主行为中的大脑活动和结构动力学，进一步推动了这一探索。在这篇简短的综述中，我们总结了微型荧光显微镜在神经科学中的最新进展，主要集中在两种主流解决方案 - 微型单光子显微镜和微型双光子显微镜。我们讨论了它们的技术优势和局限性，以及未来改进的未满足挑战。还提供了初步应用的示例，以反映涉及身体运动、长而复杂的方案甚至疾病进展和衰老的实验范式中脑成像的新趋势。
