Waitt Advanced Biophotonics Center, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Electrical and Computer Engineering Graduate Program, University of California, San Diego, La Jolla, CA, USA.
Nat Biotechnol. 2023 Dec;41(12):1729-1733. doi: 10.1038/s41587-023-01700-3. Epub 2023 Mar 6.
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm field of view, ~3- to 4-μm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments.
脊髓回路在传递疼痛方面起着至关重要的作用，但在行为小鼠的脊髓节内和节间的潜在活动模式仍然难以捉摸。我们开发了一种带有 7.9mm 视场、~3-4μm 横向分辨率、2.7mm 工作距离和<10g 总重量的可穿戴宽场显微镜，并表明高度局部化的机械疼痛刺激会引发多个脊髓节段广泛协调的星形胶质细胞兴奋。
