在行为小鼠的脊髓中进行节段间成像。
Trans-segmental imaging in the spinal cord of behaving mice.
机构信息
Waitt Advanced Biophotonics Center, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Electrical and Computer Engineering Graduate Program, University of California, San Diego, La Jolla, CA, USA.
出版信息
Nat Biotechnol. 2023 Dec;41(12):1729-1733. doi: 10.1038/s41587-023-01700-3. Epub 2023 Mar 6.
Abstract
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm field of view, ~3- to 4-μm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments.
摘要
脊髓回路在传递疼痛方面起着至关重要的作用，但在行为小鼠的脊髓节内和节间的潜在活动模式仍然难以捉摸。我们开发了一种带有 7.9mm 视场、~3-4μm 横向分辨率、2.7mm 工作距离和<10g 总重量的可穿戴宽场显微镜，并表明高度局部化的机械疼痛刺激会引发多个脊髓节段广泛协调的星形胶质细胞兴奋。
相似文献
1
Trans-segmental imaging in the spinal cord of behaving mice.在行为小鼠的脊髓中进行节段间成像。
Nat Biotechnol. 2023 Dec;41(12):1729-1733. doi: 10.1038/s41587-023-01700-3. Epub 2023 Mar 6.
2
Multiplex translaminar imaging in the spinal cord of behaving mice.在行为小鼠的脊髓中进行多重层间成像。
Nat Commun. 2023 Mar 21;14(1):1427. doi: 10.1038/s41467-023-36959-2.
3
Miniaturized photoacoustic microscope for multi-segmental spinal cord imaging in freely moving mice.用于自由活动小鼠多节段脊髓成像的微型光声显微镜。
Opt Lett. 2024 Oct 15;49(20):5909-5912. doi: 10.1364/OL.537449.
4
Differential fMRI Activation Patterns to Noxious Heat and Tactile Stimuli in the Primate Spinal Cord.灵长类动物脊髓对有害热刺激和触觉刺激的功能性磁共振成像激活模式差异
J Neurosci. 2015 Jul 22;35(29):10493-502. doi: 10.1523/JNEUROSCI.0583-15.2015.
5
Imaging spinal cord activity in behaving animals.在活动动物中对脊髓活动进行成像。
Exp Neurol. 2019 Oct;320:112974. doi: 10.1016/j.expneurol.2019.112974. Epub 2019 Jun 6.
6
Imaging large-scale cellular activity in spinal cord of freely behaving mice.在自由活动的小鼠脊髓中对大规模细胞活动进行成像。
Nat Commun. 2016 Apr 28;7:11450. doi: 10.1038/ncomms11450.
7
The roles of spatial recruitment and discharge frequency in spinal cord coding of pain: a combined electrophysiological and imaging investigation.空间募集和放电频率在脊髓疼痛编码中的作用：一项电生理与成像相结合的研究。
Pain. 1993 Jun;53(3):295-309. doi: 10.1016/0304-3959(93)90226-F.
8
Identification of spinal circuits transmitting and gating mechanical pain.识别传递和控制机械性疼痛的脊髓回路。
Cell. 2014 Dec 4;159(6):1417-1432. doi: 10.1016/j.cell.2014.11.003. Epub 2014 Nov 20.
9
Assessing the spatial distribution of cervical spinal cord activity during tactile stimulation of the upper extremity in humans with functional magnetic resonance imaging.应用功能磁共振成像评估人类上肢触觉刺激时颈脊髓活动的空间分布。
Neuroimage. 2020 Aug 15;217:116905. doi: 10.1016/j.neuroimage.2020.116905. Epub 2020 May 6.
10
Colocalization of aromatase in spinal cord astrocytes: differences in expression and relationship to mechanical and thermal hyperalgesia in murine models of a painful and a non-painful bone tumor.芳香化酶在脊髓星形胶质细胞中的共定位：在疼痛性和非疼痛性骨肿瘤小鼠模型中表达的差异及其与机械性和热痛觉过敏的关系
Neuroscience. 2015 Aug 20;301:235-45. doi: 10.1016/j.neuroscience.2015.06.009. Epub 2015 Jun 10.
引用本文的文献
1
3D-printed weight holders design and testing in mouse models of spinal cord injury.3D打印重物固定器在脊髓损伤小鼠模型中的设计与测试
Front Drug Deliv. 2024 May 22;4:1397056. doi: 10.3389/fddev.2024.1397056. eCollection 2024.
2
Reliability of task-based fMRI in the dorsal horn of the human spinal cord.基于任务的功能磁共振成像在人类脊髓背角的可靠性
Imaging Neurosci (Camb). 2024 Aug 22;2. doi: 10.1162/imag_a_00273. eCollection 2024.
3
Differential modification of ascending spinal outputs in acute and chronic pain states.急性和慢性疼痛状态下脊髓上行输出的差异性修饰
Neuron. 2025 Apr 16;113(8):1223-1239.e5. doi: 10.1016/j.neuron.2025.01.031. Epub 2025 Feb 28.
4
Long-term optical imaging of the spinal cord in awake behaving mice.清醒行为小鼠脊髓的长期光学成像
Nat Methods. 2024 Dec;21(12):2363-2375. doi: 10.1038/s41592-024-02476-3. Epub 2024 Nov 12.
5
A multichannel electrophysiological approach to noninvasively and precisely record human spinal cord activity.一种多通道电生理方法，可无创、精确地记录人体脊髓活动。
PLoS Biol. 2024 Oct 31;22(10):e3002828. doi: 10.1371/journal.pbio.3002828. eCollection 2024 Oct.
6
Neuronal maturation and axon regeneration: unfixing circuitry to enable repair.神经元成熟和轴突再生：解除固定的电路以实现修复。
Nat Rev Neurosci. 2024 Oct;25(10):649-667. doi: 10.1038/s41583-024-00849-3. Epub 2024 Aug 20.
7
Fast, Accurate, and Versatile Data Analysis Platform for the Quantification of Molecular Spatiotemporal Signals.用于定量分析分子时空信号的快速、准确且通用的数据分析平台。
bioRxiv. 2024 Jun 1:2024.05.02.592259. doi: 10.1101/2024.05.02.592259.
8
Reliability of task-based fMRI in the dorsal horn of the human spinal cord.基于任务的功能磁共振成像在人类脊髓背角的可靠性
bioRxiv. 2024 Jun 25:2023.12.22.572825. doi: 10.1101/2023.12.22.572825.
9
Advances of optical miniscopes for imaging of neural activity in freely moving animals.用于自由活动动物神经活动成像的光学微型显微镜的进展
Front Neurosci. 2022 Sep 7;16:994079. doi: 10.3389/fnins.2022.994079. eCollection 2022.
10
Neurophotonic tools for microscopic measurements and manipulation: status report.用于微观测量和操作的神经光子学工具：现状报告。
Neurophotonics. 2022 Jan;9(Suppl 1):013001. doi: 10.1117/1.NPh.9.S1.013001. Epub 2022 Apr 27.
本文引用的文献
1
Astrocytes and Microglia Exhibit Cell-Specific Ca Signaling Dynamics in the Murine Spinal Cord.星形胶质细胞和小胶质细胞在小鼠脊髓中表现出细胞特异性钙信号动力学。
Front Mol Neurosci. 2022 Mar 30;15:840948. doi: 10.3389/fnmol.2022.840948. eCollection 2022.
2
Astrocytes contribute to pain gating in the spinal cord.星形胶质细胞在脊髓的疼痛闸门机制中发挥作用。
Sci Adv. 2021 Nov 5;7(45):eabi6287. doi: 10.1126/sciadv.abi6287. Epub 2021 Nov 3.
3
Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice.用于自由活动小鼠全皮层介观成像的微型头戴式显微镜。
Nat Methods. 2021 Apr;18(4):417-425. doi: 10.1038/s41592-021-01104-8. Epub 2021 Apr 5.
4
Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity.浅层脊髓星形胶质细胞调控脑干下行通路对机械感觉过敏的作用。
Nat Neurosci. 2020 Nov;23(11):1376-1387. doi: 10.1038/s41593-020-00713-4. Epub 2020 Oct 5.
5
Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals.微型荧光显微镜用于在自由活动的动物中成像大脑活动。
Neurosci Bull. 2020 Oct;36(10):1182-1190. doi: 10.1007/s12264-020-00561-z. Epub 2020 Aug 14.
6
Neural Stem Cell Grafts Form Extensive Synaptic Networks that Integrate with Host Circuits after Spinal Cord Injury.神经干细胞移植后可形成广泛的突触网络，并与脊髓损伤后的宿主回路整合。
Cell Stem Cell. 2020 Sep 3;27(3):430-440.e5. doi: 10.1016/j.stem.2020.07.007. Epub 2020 Aug 5.
7
Imaging spinal cord activity in behaving animals.在活动动物中对脊髓活动进行成像。
Exp Neurol. 2019 Oct;320:112974. doi: 10.1016/j.expneurol.2019.112974. Epub 2019 Jun 6.
8
Circuit Investigations With Open-Source Miniaturized Microscopes: Past, Present and Future.开源小型显微镜的电路研究：过去、现在与未来
Front Cell Neurosci. 2019 Apr 5;13:141. doi: 10.3389/fncel.2019.00141. eCollection 2019.
9
CaImAn an open source tool for scalable calcium imaging data analysis.CaImAn 是一个开源的工具，用于可扩展的钙成像数据分析。
Elife. 2019 Jan 17;8:e38173. doi: 10.7554/eLife.38173.
10
Imaging Cortical Dynamics in GCaMP Transgenic Rats with a Head-Mounted Widefield Macroscope.使用头戴式广角显微镜对 GCaMP 转基因大鼠进行皮质动力学成像。
Neuron. 2018 Dec 5;100(5):1045-1058.e5. doi: 10.1016/j.neuron.2018.09.050. Epub 2018 Oct 25.
Zotero 插件