Bundza A, Afshar A, Dukes T W, Myers D J, Dulac G C, Becker S A
Agriculture Canada, Animal Diseases Research Institute, NEPEAN, Ontario.
Can J Vet Res. 1988 Jan;52(1):46-52.
In order to study the pathomorphology and immunohistochemistry of peste des petits ruminants, four goats and two sheep were inoculated intranasally with the Malig-Yemen strain of peste des petits ruminants virus. The animals developed fever, nasal discharge, oral erosions, cough and diarrhea. One goat and one sheep died and one moribund goat was killed. Three animals survived the infection. At necropsy, erosive stomatitis, pneumonia and gastroenteritis were found. Histopathologically the pneumonocytes and epithelial cells of the ileum had eosinophilic cytoplasmic and nuclear inclusions. By an indirect immunoperoxidase method, the nuclei and cytoplasm of the ileal epithelial cells of one goat contained positively (brown) stained antigen, which corresponded to viral nucleocapsids by electron microscopy. Virus appeared to be released through the microvilli of the epithelial cells. We also confirmed the formation of giant cells due to peste des petits ruminants virus.
为研究小反刍兽疫的病理形态学和免疫组织化学，对4只山羊和2只绵羊经鼻接种小反刍兽疫病毒的马利格-也门株。动物出现发热、鼻液、口腔糜烂、咳嗽和腹泻。1只山羊和1只绵羊死亡，1只濒死山羊被处死。3只动物感染后存活。尸检时，发现有糜烂性口炎、肺炎和胃肠炎。组织病理学检查显示，肺细胞和回肠上皮细胞有嗜酸性细胞质和核内包涵体。通过间接免疫过氧化物酶法，1只山羊回肠上皮细胞的细胞核和细胞质含有阳性（棕色）染色抗原，通过电子显微镜观察，该抗原与病毒核衣壳相对应。病毒似乎通过上皮细胞的微绒毛释放。我们还证实了小反刍兽疫病毒可导致巨细胞形成。
