Damera Deepthi Priyanka, Manimaran Raghuraman, Krishna Venuganti Venkata Vamsi, Nag Amit
Department of Chemistry, BITS-Pilani, Hyderabad Campus, Hyderabad 500078, India.
Department of Pharmacy, BITS-Pilani, Hyderabad Campus, Hyderabad 500078, India.
ACS Omega. 2020 Jul 30;5(31):19905-19918. doi: 10.1021/acsomega.0c03148. eCollection 2020 Aug 11.
Full-color fluorescent carbon nanoparticles (CNPs) are produced by a facile and green hydrothermal method followed by the differential washing technique. twigs are used as a precursor to synthesize multiemissive light blue, blue, green, and red CNPs. Brilliant Blue FCF (BB) is a widely used synthetic food colorant, which is toxic for the human body, when consumed beyond the permitted limit. Herein, we demonstrate light blue CNPs as a sensor for selective and sensitive detection of BB a fluorescence quenching mechanism with a limit of detection of 200 nM. Temperature-dependent fluorescence and H NMR studies confirmed the mechanism as combined dynamic and static quenching. To demonstrate the practical efficacy of the sensor, BB is effectively detected and estimated in selected food samples procured from the market. Moreover, the biocompatibility of light blue and blue CNPs is examined and confirmed by performing a cytotoxicity assay on MDA-MB-231 cell lines. Subsequently, the cellular imaging study is also carried out to explore the internalization process of the CNPs as a function of concentration. To the best of our knowledge, this is the first time that twigs, a natural source of high abundance, are used as raw materials and valorized for sensing artificial food color and bioimaging purposes.
全色荧光碳纳米颗粒（CNPs）通过简便的绿色水热法结合差示洗涤技术制备。树枝被用作前驱体来合成多发射的浅蓝色、蓝色、绿色和红色CNPs。亮蓝FCF（BB）是一种广泛使用的合成食用色素，当摄入量超过允许限量时对人体有毒。在此，我们展示了浅蓝色CNPs作为一种用于选择性和灵敏检测BB的传感器，其荧光猝灭机制的检测限为200 nM。温度依赖性荧光和核磁共振氢谱研究证实该机制为动态猝灭和静态猝灭相结合。为了证明该传感器的实际功效，对从市场采购的选定食品样品中的BB进行了有效检测和估算。此外，通过对MDA-MB-231细胞系进行细胞毒性试验，检测并确认了浅蓝色和蓝色CNPs的生物相容性。随后，还进行了细胞成像研究，以探索CNPs作为浓度函数的内化过程。据我们所知，这是首次将高丰度的天然来源树枝用作原材料，并将其用于检测人工食用色素和生物成像目的。
