Gao Yu, Huang Siyuan, Wang Yujie, Lin Hongxin, Pan Zhiyong, Zhang Shubao, Zhang Jie, Wang Wenquan, Cheng Shanhan, Chen Yinhua
Sanya Nanfan Research Institute of Hainan University, School of Life Science, Hainan University, Haikou, Hainan, China.
College of Tropical Crops, Hainan University, Haikou, Hainan, China.
Front Plant Sci. 2023 Mar 7;14:1130924. doi: 10.3389/fpls.2023.1130924. eCollection 2023.
Plants and arbuscular mycorrhizal fungi (AMF) mutualistic interactions are essential for sustainable agriculture production. Although it is shown that AMF inoculation improves cassava physiological performances and yield traits, the molecular mechanisms involved in AM symbiosis remain largely unknown. Herein, we integrated metabolomics and transcriptomics analyses of symbiotic (Ri) and asymbiotic (CK) cassava roots and explored AM-induced biochemical and transcriptional changes.
Three weeks (3w) after AMF inoculations, proliferating fungal hyphae were observable, and plant height and root length were significantly increased. In total, we identified 1,016 metabolites, of which 25 were differentially accumulated (DAMs) at 3w. The most highly induced metabolites were 5-aminolevulinic acid, L-glutamic acid, and lysoPC 18:2. Transcriptome analysis identified 693 and 6,481 differentially expressed genes (DEGs) in the comparison between CK (3w) against Ri at 3w and 6w, respectively. Functional enrichment analyses of DAMs and DEGs unveiled transport, amino acids and sugar metabolisms, biosynthesis of secondary metabolites, plant hormone signal transduction, phenylpropanoid biosynthesis, and plant-pathogen interactions as the most differentially regulated pathways. Potential candidate genes, including nitrogen and phosphate transporters, transcription factors, phytohormone, sugar metabolism-related, and SYM (symbiosis) signaling pathway-related, were identified for future functional studies.
Our results provide molecular insights into AM symbiosis and valuable resources for improving cassava production.
植物与丛枝菌根真菌（AMF）的共生相互作用对可持续农业生产至关重要。尽管已表明接种AMF可改善木薯的生理性能和产量性状，但AM共生所涉及的分子机制仍 largely未知。在此，我们整合了共生（Ri）和非共生（CK）木薯根的代谢组学和转录组学分析，并探索了AM诱导的生化和转录变化。
接种AMF三周（3w）后，可观察到真菌菌丝增殖，株高和根长显著增加。我们总共鉴定出1016种代谢物，其中25种在3w时差异积累（DAMs）。诱导程度最高的代谢物是5-氨基乙酰丙酸、L-谷氨酸和溶血磷脂酰胆碱18:2。转录组分析在CK（3w）与3w和6w时的Ri的比较中分别鉴定出693个和6481个差异表达基因（DEGs）。对DAMs和DEGs的功能富集分析揭示了转运、氨基酸和糖代谢、次生代谢物生物合成、植物激素信号转导、苯丙烷生物合成以及植物-病原体相互作用是差异调节最显著的途径。鉴定出了包括氮和磷转运体、转录因子、植物激素、糖代谢相关以及共生（SYM）信号通路相关的潜在候选基因，以供未来进行功能研究。
我们的结果为AM共生提供了分子见解，并为提高木薯产量提供了宝贵资源。
