Current approaches for detection of hydrogen sulfide and persulfidation in biological systems.

The past decades have witnessed hydrogen sulfide (HS) serving as gaseous signaling molecule participating in diverse cellular and physiological processes in biological systems. Recently, a considerable number of studies highlight the signaling role of this redox-regulating molecule occurs via persulfidation, which is a post-translation modification of protein cysteine residues by covalent addition of thiol group form persulfide. However, our current understanding on detection of HS and persulfidation in biological systems still lags behind. This review aims to summarize current approaches for measuring HS and persulfidated levels in biological systems. Meanwhile, potential interference may exist in plant research has been proposed and discussed.