Department of Chemistry, College of Sciences, University of Ha'il, Ha'il 2440, Saudi Arabia.
Molecular Diagnostic and Personalised Therapeutics Unit, University of Ha'il, Ha'il 2440, Saudi Arabia.
Molecules. 2020 Aug 12;25(16):3675. doi: 10.3390/molecules25163675.
Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance in the elderly. This study aims to understand links among aging, gluco-oxidative stress, and autoantibodies in asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemical changes. Significant inhibition of advanced glycation end products (AGEs) formation was achieved using garlic extract (53.75%) and epigallocatechin-3-gallate from green tea (72.5%). Increased amounts of serum carbonyl content (2.42 ± 0.5) and pentosidine (0.0321 ± 0.0029) were detected in IV-S (S represent smokers) vs. IV group individuals. Direct binding ELISA results exhibited significantly high autoantibodies against Gly-HSA in group IV-S (0.55 ± 0.054; < 0.001) and III-S (0.40 ± 0.044; < 0.01) individuals as compared to the age matched subjects who were non-smokers (group IV and III). Moreover, high average percent inhibition (51.3 ± 4.1%) was obtained against Gly-HSA in IV-S group individuals. Apparent association constant was found to be high for serum immunoglobulin-G (IgG) from group IV-S (1.18 × 10 M) vs. serum IgG from IV group (3.32 × 10 M). Aging induced gluco-oxidative stress and AGEs formation may generate neo-epitopes on blood-proteins, contributing to production of autoantibodies in the elderly, especially smokers. Use of anti-glycation natural products may reduce age-related pathophysiological changes.
衰老是自由基、抗氧化剂和老年人免疫失衡的逐渐变化。本研究旨在了解无症状个体衰老、糖氧化应激和自身抗体之间的联系。人血清白蛋白的体外糖化（Gly-HSA）诱导明显的生化变化。大蒜提取物（53.75%）和绿茶表没食子儿茶素-3-没食子酸酯（72.5%）可显著抑制晚期糖基化终产物（AGEs）的形成。在 IV-S（S 代表吸烟者）组个体中，检测到血清羰基含量（2.42±0.5）和戊糖素（0.0321±0.0029）的含量增加。与非吸烟者（IV 和 III 组）相比，IV-S（0.55±0.054；<0.001）和 III-S（0.40±0.044；<0.01）组个体针对 Gly-HSA 的自身抗体明显较高。与 IV 组相比，IV-S 组个体针对 Gly-HSA 的平均抑制率（51.3±4.1%）较高。发现 IV-S 组血清免疫球蛋白-G（IgG）的表观缔合常数明显高于 IV 组（1.18×10 M 对 3.32×10 M）。衰老诱导的糖氧化应激和 AGEs 的形成可能在血液蛋白上产生新的表位，导致老年人，特别是吸烟者产生自身抗体。使用抗糖化天然产物可能会减少与年龄相关的病理生理变化。
