Mattioni Marchetti Vittoria, Bitar Ibrahim, Piazza Aurora, Mercato Alessandra, Fogato Elena, Hrabak Jaroslav, Migliavacca Roberta
Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, 32300 Pilsen, Czech Republic.
Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic.
Microorganisms. 2020 Aug 12;8(8):1232. doi: 10.3390/microorganisms8081232.
: VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and is able to hydrolyze all beta-lactams antibiotics, except for monobactams, and including carbapenems. Here we characterize a VIM-producing IncA plasmid isolated from a clinical ST69 strain from an Italian Long-Term Care Facility (LTCF) inpatient. : An antimicrobial susceptibility test and conjugation assay were carried out, and the transferability of the gene was confirmed in the transconjugant. Whole-genome sequencing (WGS) of the strain 550 was performed using the Sequel I platform. Genome assembly was performed using "Microbial Assembly". Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases. Assembly resulted in three complete circular contigs: the chromosome (4,962,700 bp), an IncA plasmid (p550_IncA_VIM_1; 162,608 bp), harboring genes coding for aminoglycoside resistance (, , , , ), beta-lactam resistance (, ), macrolides resistance (), phenicol resistance (), quinolones resistance (), sulphonamide resistance (, ), and trimethoprim resistance (), and an IncK/Z plasmid (p550_IncB_O_K_Z; 100,306 bp), free of antibiotic resistance genes. The increase in reports of IncA plasmids bearing different antimicrobial resistance genes highlights the overall important role of IncA plasmids in disseminating carbapenemase genes, with a preference for the gene in Italy.
VIM（维罗纳整合子编码金属β-内酰胺酶）是金属β-内酰胺酶（MBLs）家族的一员，能够水解除单环β-内酰胺类抗生素外的所有β-内酰胺类抗生素，包括碳青霉烯类。在此，我们对从意大利一家长期护理机构（LTCF）住院患者的临床ST69菌株中分离出的产VIM的IncA质粒进行了表征。进行了药敏试验和接合试验，并在接合子中证实了该基因的可转移性。使用Sequel I平台对菌株550进行了全基因组测序（WGS）。使用“微生物组装”进行基因组组装。通过将重叠群上传到ResFinder和PlasmidFinder数据库进行基因组分析。组装产生了三个完整的环状重叠群：染色体（4,962,700 bp）、一个IncA质粒（p550_IncA_VIM_1；162,608 bp），其携带编码氨基糖苷类耐药性（、、、、）、β-内酰胺耐药性（、）、大环内酯类耐药性（）、氯霉素耐药性（）、喹诺酮类耐药性（）、磺胺类耐药性（、）和甲氧苄啶耐药性（）的基因，以及一个IncK/Z质粒（p550_IncB_O_K_Z；100,306 bp），该质粒不含抗生素耐药基因。携带不同抗菌耐药基因的IncA质粒报告数量的增加凸显了IncA质粒在传播碳青霉烯酶基因方面的总体重要作用，在意大利对基因有偏好。
