Complete Genome and Plasmids Sequences of a Clinical Isolate Producing Plasmid Mediated NDM-1 from Italy.

The spread of carbapenemase genes, such as , in poses a public health threat. The aim of the study was to characterize the genome and plasmids sequences of an NDM-1-positive strain (IBCRE14), which was isolated in 2019 from a catheterized patient hospitalized in Italy. : Whole genome sequencing (WGS) of IBCRE14 was performed on extracted genomic DNA using Sequel I platform. Genome assembly was performed using "Microbial Assembly". Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases from the Center for Genomic Epidemiology. IBCRE14 had a genome size of 4,018,329 bp and harboured genes coding for resistance to aminoglycosides (), phenicol (), tetracycline (), and trimethoprim (). A large plasmid (pIB_NDM_1) harboured antibiotic resistance genes against sulphonamide (), trimethoprim (), tetracycline (), rifampicin (), aminoglycosides (), and beta-lactams (, ). Furthermore, a small plasmid (pIB_COL3M) harboured a gene coding for quinolone resistance. The ability to conjugate and the presence of a composite antibiotic resistance island suggests that pIB_NDM_1 could both acquire more resistance genes and easily disseminate. To our knowledge, this is the first report on an untypable plasmid harbouring in in Italy.