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一种收集和安装环氧树脂包埋标本带状连续切片的简单方法。

A simple method for collecting and mounting ribboned serial sections of epoxy embedded specimens.

作者信息

Royer S M

机构信息

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.

出版信息

Stain Technol. 1988 Jan;63(1):23-6. doi: 10.3109/10520298809107155.

Abstract

A simple and rapid method of handling ribboned serial sections of epoxy embedded specimens is described. Ribbons are cut from a block having the leading and trailing sides coated with contact cement. A scoop made from polyethylene tubing is used to remove a ribbon of sections from the boat of a glass or diamond knife and to transfer it to a pool of water on a microscope slide. Many ribbons (comprising hundreds of sections) can be mounted on a single slide. This method requires the construction of only one simple, inexpensive tool, the polyethylene scoop, and otherwise utilizes only items commonly available in the laboratory.

摘要

本文描述了一种处理环氧树脂包埋标本带状连续切片的简单快速方法。切片带是从一个前后侧面涂有接触胶的蜡块上切下来的。用聚乙烯管制成的勺子从玻璃刀或钻石刀的水槽中取出一条切片带,并将其转移到载玻片上的一滩水中。许多切片带(包含数百个切片)可以安装在一张载玻片上。这种方法只需要制作一个简单、廉价的工具——聚乙烯勺子,并且在其他方面仅使用实验室中常见的物品。

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