Department of Pharmacology, College of Medicine, Inha University, Incheon 22212, Korea.
Hypoxia-Related Diseases Research Center, College of Medicine, Inha University, Incheon 22212, Korea.
Int J Mol Sci. 2020 Aug 14;21(16):5842. doi: 10.3390/ijms21165842.
Although Alzheimer's disease (AD)-like pathology is frequently found in patients with post-stroke dementia, little is known about the effects of aerobic exercise on the modifications of tau and related proteins. Therefore, we evaluated the effects of aerobic exercise on the phosphorylation and acetylation of tau and the expressions of tau-related proteins, after middle cerebral artery occlusion (MCAO) stroke. Twenty-four Sprague-Dawley rats with MCAO infarction were used in this study. The rehabilitation group (RG) received treadmill training 40 min/day for 12 weeks, whereas the sedentary group (SG) did not receive any type of training. Functional tests, such as the single pellet reaching task, rotarod, and radial arm maze tests, were performed monthly for 3 months. In ipsilateral cortices in the RG and SG groups, level of Ac-tau was lower in the RG, whereas levels of p-tau, p-tau, and p-tau were not significantly lower in the RG. Level of phosphorylated glycogen synthase kinase 3-beta Tyr 216 (p-GSK3β) was lower in the RG, but levels of p-AMPK and phosphorylated glycogen synthase kinase 3-beta Ser 9 (p-GSK3β) were not significantly lower. Levels of COX-2 and BDNF were not significantly different between the two groups, while SIRT1 significantly decreased in ipsilateral cortices in RG. In addition, aerobic training also improved motor, balance, and memory functions. Rehabilitation with aerobic exercise inhibited tau modification, especially tau acetylation, following infarction in the rat MCAO model, which was accompanied with the improvement of motor and cognitive functions.
尽管在患有中风后痴呆的患者中经常发现阿尔茨海默病（AD）样病理学，但对于有氧运动对tau 和相关蛋白的修饰的影响知之甚少。因此，我们评估了有氧运动对大脑中动脉闭塞（MCAO）中风后 tau 的磷酸化和乙酰化以及tau 相关蛋白表达的影响。本研究使用了 24 只 MCAO 梗塞的 Sprague-Dawley 大鼠。康复组（RG）接受了 12 周每天 40 分钟的跑步机训练，而对照组（SG）则没有接受任何类型的训练。在第 3 个月，每月进行功能测试，例如单球触及任务、转棒和放射臂迷宫测试。在 RG 和 SG 组的同侧皮质中，RG 中的 Ac-tau 水平较低，而 RG 中的 p-tau、p-tau 和 p-tau 水平没有明显降低。RG 中的磷酸化糖原合成酶激酶 3-β Tyr 216（p-GSK3β）水平较低，但 p-AMPK 和磷酸化糖原合成酶激酶 3-β Ser 9（p-GSK3β）水平没有明显降低。两组 COX-2 和 BDNF 水平无显著差异，而 RG 中的 SIRT1 显著降低。此外，有氧运动训练还改善了运动、平衡和记忆功能。有氧康复运动抑制了 tau 的修饰，特别是 tau 的乙酰化，在大鼠 MCAO 模型中的梗塞后，这伴随着运动和认知功能的改善。
