Baluta Sylwia, Lesiak Anna, Cabaj Joanna
Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Sensors (Basel). 2020 Aug 14;20(16):4567. doi: 10.3390/s20164567.
Although neurotransmitters are present in human serum at the nM level, any dysfunction of the catecholamines concentration may lead to numerous serious health problems. Due to this fact, rapid and sensitive catecholamines detection is extremely important in modern medicine. However, there is no device that would measure the concentration of these compounds in body fluids. The main goal of the present study is to design a simple as possible, cost-effective new biosensor-based system for the detection of neurotransmitters, using nontoxic reagents. The miniature Au-E biosensor was designed and constructed through the immobilization of tyrosinase on an electroactive layer of cysteamine and carbon nanoparticles covering the gold electrode. This sensing arrangement utilized the catalytic oxidation of norepinephrine (NE) to NE quinone, measured with voltammetric techniques: cyclic voltammetry and differential pulse voltammetry. The prepared bio-system exhibited good parameters: a broad linear range (1-200 μM), limit of detection equal to 196 nM, limit of quantification equal to 312 nM, and high selectivity and sensitivity. It is noteworthy that described method was successfully applied for NE determination in real samples.
尽管神经递质在人血清中的浓度处于纳摩尔水平，但儿茶酚胺浓度的任何功能障碍都可能导致许多严重的健康问题。基于这一事实，快速灵敏地检测儿茶酚胺在现代医学中极为重要。然而，目前尚无能够测量体液中这些化合物浓度的设备。本研究的主要目标是设计一种尽可能简单、具有成本效益的新型基于生物传感器的系统，用于使用无毒试剂检测神经递质。通过将酪氨酸酶固定在覆盖金电极的半胱胺和碳纳米颗粒的电活性层上，设计并构建了微型金 - E生物传感器。这种传感装置利用伏安技术（循环伏安法和差分脉冲伏安法）测量去甲肾上腺素（NE）催化氧化为NE醌的过程。所制备的生物系统表现出良好的参数：宽线性范围（1 - 200 μM）、检测限等于196 nM、定量限等于312 nM，以及高选择性和灵敏度。值得注意的是，所描述的方法已成功应用于实际样品中NE的测定。
