Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Int J Biol Macromol. 2020 Dec 1;164:2804-2812. doi: 10.1016/j.ijbiomac.2020.08.146. Epub 2020 Aug 21.
Herein, gellan gum (GG), a nature-derived polysaccharide, was applied to combine fluorescein isothiocyanate (FITC) to fabricate a bio-imaging material. The synthesis process of the FITC grafted GG (GG-F) and manufacturing method of GG-F scaffolds are presented. Chemical, physicochemical, and mechanical properties were characterized. In vitro study and in vivo study by implanting the GG-F scaffolds under the subcutaneous area of the nude mice were carried out to verify biocompatibility and safety of the material. The emission of the FITC was confirmed with high-resolution confocal laser scanning microscope (SR CLMS) and fluorescence in vivo imaging (FOBI). The results exhibited well-synthesized GG-F and the manufactured GG-F scaffolds showed similar property of GG scaffolds which confirms that the chemical modification does not affect the property of GG scaffolds. The in vitro and in vivo study exhibited biocompatibility of the GG-F material. Overall, the properly blended GG-F in GG did not influence the characteristics of the pristine GG except for the chemical property. Therefore, the GG-F can be applied for the future analysis in verifying the mechanism of GG characters and can be a promising candidate for bio-imaging.
在此，我们使用来源于自然界的多糖——结冷胶（GG），将其与荧光素异硫氰酸酯（FITC）结合，制备生物成像材料。本文介绍了 FITC 接枝 GG（GG-F）的合成过程以及 GG-F 支架的制造方法，并对其化学、物理化学和机械性能进行了表征。通过将 GG-F 支架植入裸鼠皮下区域进行体外和体内研究，验证了材料的生物相容性和安全性。利用高分辨率共聚焦激光扫描显微镜（SR CLMS）和荧光体内成像（FOBI）确认了 FITC 的发射。结果表明，成功合成了 GG-F，所制造的 GG-F 支架具有与 GG 支架相似的性能，这证实了化学修饰不会影响 GG 支架的性能。体外和体内研究均表明 GG-F 材料具有良好的生物相容性。总的来说，在 GG 中适当混合的 GG-F 不会影响原始 GG 的特性，除了化学性质。因此，GG-F 可以应用于未来分析中，以验证 GG 特性的机制，并且可能成为生物成像的有前途的候选材料。
