Yao Wan-Zi, Veeraperumal Suresh, Qiu Hua-Mai, Chen Xian-Qiang, Cheong Kit-Leong
Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, 515063 Guangdong People's Republic of China.
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, 530200 Guangxi China.
3 Biotech. 2020 Sep;10(9):386. doi: 10.1007/s13205-020-02379-y. Epub 2020 Aug 11.
In this study, the anticancer effects of polysaccharides (PHPs) on human colon cancer cells and non-cancerous cells were evaluated. PHP was extracted by an ultrasonic/microwave-assisted method, and three fractions of polysaccharides (PHP-F1, PHP-F2 and PHP-F3) were obtained through a DEAE-52 cellulose ion-exchange column. The results of the cytotoxicity test showed that all of the PHP fractions had inhibitory effects on the growth of colon cancer cells HT-29, LoVo and SW-480, but no toxic effects on the normal human cells HaCaT. The fractions PHP-F2 and PHP-F3 had the most significant cytotoxicity on HT-29 cells. Studies on intracellular reactive oxygen species (ROS) levels, cell apoptosis, the apoptosis index (using Hoechst 33342 staining) and analysis of cell cycle arrest using flow cytometry revealed that the fractions PHP-F2 and PHP-F3 could apparently induce oxidative stress and apoptosis in HT-29 cells and cause cell G0-G1 phase arrest. These findings suggest that polysaccharides from have anticancer effects on human colon cancer cells and therefore might be regarded as new candidates for the prevention and treatment of colon cancers.
在本研究中，评估了多糖（PHPs）对人结肠癌细胞和非癌细胞的抗癌作用。采用超声/微波辅助法提取PHP，并通过DEAE-52纤维素离子交换柱获得了三种多糖组分（PHP-F1、PHP-F2和PHP-F3）。细胞毒性试验结果表明，所有PHP组分均对结肠癌细胞HT-29、LoVo和SW-480的生长具有抑制作用，但对正常人细胞HaCaT无毒性作用。PHP-F2和PHP-F3组分对HT-29细胞的细胞毒性最为显著。对细胞内活性氧（ROS）水平、细胞凋亡、凋亡指数（使用Hoechst 33342染色）以及使用流式细胞术分析细胞周期阻滞的研究表明，PHP-F2和PHP-F3组分可明显诱导HT-29细胞发生氧化应激和凋亡，并导致细胞G0-G1期阻滞。这些发现表明，[来源]的多糖对人结肠癌细胞具有抗癌作用，因此可能被视为预防和治疗结肠癌的新候选药物。
