Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou 515063, Guangdong, PR China.
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China.
Carbohydr Polym. 2020 Oct 15;246:116626. doi: 10.1016/j.carbpol.2020.116626. Epub 2020 Jun 15.
This study examined the beneficial effects of porphyran from Porphyra haitanensis (PHP) on intestinal epithelial cells, in terms of cell proliferation and migration and elucidated the potential molecular mechanism of action of PHP. Purified PHP is a homogenous polysaccharide with a molecular weight of 2.01 × 10 Da, intrinsic viscosity [η] of 463.76 mL/g, and radius of gyration of 61.2 nm. When the intestinal epithelial wound healing activity of PHP was investigated in vitro using the IEC-6 cell line (intestinal epithelial cells-6), it was found that PHP could promote cell migration and proliferation. PHP enhanced the protein expression of cell division control protein 42, paxillin, and focal adhesion kinase, which suggest that PHP might modulate the expression of these proteins to improve intestinal epithelial healing. Thus, this study indicated that PHP could serve as a potential source of functional food constituents for intestinal epithelial protection and restoration.
本研究考察了来自坛紫菜（PHP）的岩藻黄质对肠道上皮细胞的有益影响，包括细胞增殖和迁移，并阐明了 PHP 的潜在作用机制。纯化的 PHP 是一种均一的多糖，分子量为 2.01×10 Da，特性黏度 [η]为 463.76 mL/g，回转半径为 61.2 nm。当使用 IEC-6 细胞系（肠上皮细胞-6）在体外研究 PHP 的肠道上皮细胞愈合活性时，发现 PHP 可以促进细胞迁移和增殖。PHP 增强了细胞分裂控制蛋白 42、桩蛋白和粘着斑激酶的蛋白表达，这表明 PHP 可能通过调节这些蛋白的表达来改善肠道上皮愈合。因此，本研究表明，PHP 可作为肠道上皮保护和修复的功能性食品成分的潜在来源。
