Balow J E, Parrillo J E, Fauci A S
Immunology. 1977 Jun;32(6):899-904.
The direct immunosuppressive effects of cyclophosphamide were examined by incubating normal guinea-pig mononuclear cells with activated cyclophosphamide prepared from the serum of separate animals which were treated with cyclophosphamide in vivo. Treated cells were subsequently assayed in a panel of tests of cell-mediated immunity either immediately or following recovery during variable periods of incubation. It was found that cyclophosphamide (1) induces changes in lymphocyte functions which are not due simply to cell death; (2) is markedly antiproliferative in mitogen-induced blastogenesis; (3) depresses migration inhibitory factor production only after a delay of several hours and (4) depresses certain cytotoxicity reactions immediately following treatment, such responses are subsequently normalized when the cells are allowed to recover in culture.
通过将正常豚鼠单核细胞与从体内用环磷酰胺处理的单独动物血清中制备的活化环磷酰胺一起孵育，来检测环磷酰胺的直接免疫抑制作用。随后，对处理过的细胞立即或在不同孵育时间段恢复后，进行一系列细胞介导免疫测试。结果发现，环磷酰胺：(1) 诱导淋巴细胞功能发生变化，这并非仅仅是由于细胞死亡所致；(2) 在有丝分裂原诱导的细胞增殖中具有明显的抗增殖作用；(3) 仅在延迟数小时后才抑制迁移抑制因子的产生；(4) 在处理后立即抑制某些细胞毒性反应，当细胞在培养中恢复时，这些反应随后恢复正常。
