Melnikova Nina, Vorobyova Olga, Balakireva Alyona, Malygina Darina, Solovyeva Anna, Belyaeva Kseniya, Orekhov Dmitry, Knyazev Alexander
Department of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin sq., 603950 Nizhny Novgorod, Russia.
Department of Engineering Physics and Chemistry, Nizhny Novgorod State Technical University n.a. R.E. Alekseev, 24 Minin st., 603950 Nizhny Novgorod, Russia.
Pharmaceuticals (Basel). 2020 Aug 22;13(9):207. doi: 10.3390/ph13090207.
We studied oleogels containing zinc oxide nanoparticles (ZnO NPs) and lupane triterpenoids in sunflower oil for the treatment of burns. The modification of ZnO was carried out by treatment with alcohol solutions of betulin, betulonic acid, betulin diacetate and betulin diphosphate. The properties of modified ZnO NPs were studied by powder XRD (average sizes of 10-20 nm), FTIR (νZnO 450 cm), UV-vis (345-360 nm), and blue-violet emission (380-420 nm). The identification and assay of modified ZnO NPs and triterpenoids were estimated. The treatment by oleogels of deep II-degree burns was studied on rats using histological studies, Doppler flowmetry and evaluation of enzymes activity and malonic dialdehyde (MDA) level. After the action of oleogels, burn wound area, and the necrosis decreased twice on the 10th day in comparison with the 1st day after burn. The microcirculation index in the near-wound zone by 20-30% improved compared with the group without treatment. Evaluation of the enzyme activity and the MDA level after treatment by oleogels during the course of 10 days showed them returning to normal. The improvement of antioxidant biochemical indexes, as well as wounds' healing, was mainly determined by the influence of zinc oxide nanoparticles.
我们研究了向日葵油中含有氧化锌纳米颗粒（ZnO NPs）和羽扇豆烷三萜类化合物的油凝胶用于烧伤治疗的情况。通过用桦木醇、桦木酸、桦木二乙酸酯和桦木二磷酸酯的醇溶液处理来对ZnO进行改性。通过粉末XRD（平均尺寸为10 - 20 nm）、FTIR（νZnO 450 cm）、紫外可见光谱（345 - 360 nm）和蓝紫光发射（380 - 420 nm）研究了改性ZnO NPs的性质。对改性ZnO NPs和三萜类化合物进行了鉴定和含量测定。利用组织学研究、多普勒血流仪以及对酶活性和丙二醛（MDA）水平的评估，在大鼠身上研究了油凝胶对深二度烧伤的治疗效果。在油凝胶作用后，与烧伤后第1天相比，烧伤创面面积和坏死程度在第10天减少了一半。与未治疗组相比，创面周围区域的微循环指数提高了20 - 30%。在10天的治疗过程中，对油凝胶治疗后的酶活性和MDA水平进行评估，结果显示它们恢复到了正常水平。抗氧化生化指标的改善以及伤口的愈合，主要是由氧化锌纳米颗粒的影响所决定的。
