磷酸结合蛋白对大肠杆菌原生质球中磷酸盐转运的恢复作用
Restoration of phosphate transport by the phosphate-binding protein in spheroplasts of Escherichia coli.
作者信息
Gerdes R G, Strickland K P, Rosenberg H
出版信息
J Bacteriol. 1977 Aug;131(2):512-8. doi: 10.1128/jb.131.2.512-518.1977.
Abstract
Reconstitution of phosphate transport in Escherichia coli was demonstrated. Conversion of E. coli K10 cells to spheroplasts decreased phosphate transport to about 2%. Addition of purified phosphate-binding protein at physiological levels to these spheroplasts caused a mean 14-fold increase in phosphate transport rate. Crude shock fluid fractions were also stimulatory but not if the shock fluid was obtained from mutants lacking phosphate-binding protein. The effect of the binding protein was abolished by its specific antibody. The phosphate was shown to have entered the cell, where it became esterified. Reconstitution was not possible with cold-shocked or osmotically shocked cells.
摘要
已证实大肠杆菌中磷酸盐转运的重建。将大肠杆菌K10细胞转化为原生质体可使磷酸盐转运降至约2%。向这些原生质体中添加生理水平的纯化磷酸盐结合蛋白，可使磷酸盐转运速率平均提高14倍。粗制的休克液组分也具有刺激作用，但如果休克液取自缺乏磷酸盐结合蛋白的突变体，则无此作用。结合蛋白的作用可被其特异性抗体消除。已证明磷酸盐进入了细胞并在其中被酯化。冷休克或渗透休克的细胞无法实现重建。
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