Biology Department, College of Science, Imam Mohammad ibn Saud Islamic University (IMSIU), Riyadh, 11623, Saudi Arabia.
Chemistry Department, College of Science, Imam Mohammad ibn Saud Islamic University (IMSIU), Riyadh, 11623, Saudi Arabia.
Environ Sci Pollut Res Int. 2021 Jan;28(2):1587-1595. doi: 10.1007/s11356-020-09843-5. Epub 2020 Aug 26.
Breast and colon carcinomas are two types of common cancers which lead to cancer-related deaths. Due to their cytotoxic potential against cancer cells, recently many studies of copper nanoparticles (CuNPs) have been conducted. In the current work, we aim to evaluate the cytotoxic and apoptosis-inducing effects of CuNPs on the human breast (MCF-7) and colon (LoVo) cancer cells. CuNPs were prepared in starch-stabilizing aqueous solution by electroless deposition technique in alkaline tartrate bath using formaldehyde as the reducing agent of copper sulfate. The obtained CuNPs were characterized by SEM, TEM, and XRD to confirm the particle size, morphology, and chemical composition. Standard colorimetric MTT and LDH assays were used to estimate the cytotoxic effect of CuNPs on MCF-7 and LoVo cells. Furthermore, CuNP-treated cells undergoing apoptosis were assessed based on the expression of apoptosis-related genes using qRT-PCR. The results indicate that the mean particle size of the synthesized CuNPs was ~ 50-60 nm, and they were spherical in shape with mainly the chemical structure of the copper metallic phase. MTT assay revealed that CuNPs induced cytotoxicity in tested cells with IC rates of 16.4 (in MCF-7) and 21.6 μg/ml (in LoVo). Moreover, qRT-PCR analysis showed that CuNPs caused a significant increment of Bax, P53, and Caspases 9, 8, and 3 genes. Overall, the anticancer potential of prepared CuNPs were reported through apoptotic induction which highlight the potential use of CuNPs as an efficient anticancer agent.
乳腺癌和结肠癌是两种常见的癌症类型，它们会导致与癌症相关的死亡。由于其对癌细胞的细胞毒性潜力，最近对铜纳米粒子（CuNPs）进行了许多研究。在目前的工作中，我们旨在评估 CuNPs 对人乳腺癌（MCF-7）和结肠癌（LoVo）癌细胞的细胞毒性和诱导细胞凋亡的作用。CuNPs 通过无电沉积技术在碱性酒石酸盐浴中使用甲醛作为硫酸铜的还原剂在淀粉稳定的水溶液中制备。通过 SEM、TEM 和 XRD 对所获得的 CuNPs 进行了表征，以确认粒径、形态和化学成分。标准比色 MTT 和 LDH 测定法用于估计 CuNPs 对 MCF-7 和 LoVo 细胞的细胞毒性作用。此外，基于 qRT-PCR 评估了凋亡相关基因表达的 CuNP 处理细胞的凋亡情况。结果表明，合成的 CuNPs 的平均粒径约为 50-60nm，它们呈球形，主要具有铜金属相的化学结构。MTT 测定法表明，CuNPs 以 16.4μg/ml（在 MCF-7 中）和 21.6μg/ml（在 LoVo 中）的 IC 率诱导测试细胞的细胞毒性。此外，qRT-PCR 分析表明，CuNPs 导致 Bax、P53 和 Caspases 9、8 和 3 基因的显著增加。总体而言，通过诱导细胞凋亡，报道了所制备的 CuNPs 的抗癌潜力，这突出了 CuNPs 作为有效抗癌剂的潜在用途。
