从小接种量中复苏厌氧菌：一种用于血培养研究的模型。
Recovery of anaerobic bacteria from small inocula: a model for blood culture studies.
作者信息
Collee J G, Duerden B I, Brown R
出版信息
J Clin Pathol. 1977 Jul;30(7):609-14. doi: 10.1136/jcp.30.7.609.
Abstract
The recovery of anaerobic, facultative anaerobic, and aerobic pathogens from very small inocula was studied under conditions that could be related to routine blood culture procedures. Results with Brain Heart Infusion broth were unsatisfactory. Freshly prepared Brewer thioglycollate medium gave apparently good results, but there are disadvantages when this medium is used for blood culture. Results with Difco Thiol broth were disappointing. A modification of Robertson's cooked-meat broth supplemented with Brain Heart Infusion gave good recovery and sustained viability with a wide range of test organisms including exacting strains. The findings raise points of practical importance.
摘要
在可能与常规血培养程序相关的条件下，研究了从非常小的接种物中复苏厌氧、兼性厌氧和好氧病原体的情况。脑心浸液肉汤的结果并不理想。新制备的布鲁尔硫乙醇酸盐培养基给出了明显良好的结果，但该培养基用于血培养时存在缺点。迪夫科硫醇肉汤的结果令人失望。一种补充了脑心浸液的改良罗伯逊熟肉培养基，对包括苛求菌株在内的多种测试微生物具有良好的复苏效果和持续生存能力。这些发现提出了具有实际重要性的要点。
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