Instituto de Investigación Biomédica de Málaga (IBIMA), UGC Salud Mental, Universidad de Málaga, Hospital Universitario Regional de Málaga, Málaga, Spain.
Department of Pediatrics & Pediatric Endocrinology, Hospital Infantil Universitario Niño Jesús, Universidad Autónoma de Madrid, Madrid, Spain.
Pediatr Obes. 2021 Feb;16(2):e12711. doi: 10.1111/ijpo.12711. Epub 2020 Aug 27.
In obesity adipose tissue undergoes structural re-modelling leading to a chronic low-grade inflammatory state linked to insulin resistance (IR).
We aimed to develop a clinically relevant biomarker model for stratifying IR in adolescents with obesity.
Cytokines [tumour cell derived factor 1α, monocyte chemoattract protein (MCP) 1, eotaxin and fractalkine], growth factors [brain-derived neurotrophic factor, pro-fibrotic platelet-derived growth factor (PDGF-BB) and insulin-like growth factor 1] and biochemical/metabolic factors were analysed in serum of 143 pubertal patients with obesity (50% IR; 50% non-IR) and 33 controls. Factor analysis, correlation, binary logistic regression and receiver operating characteristic analysis were used to evaluate combinations of these biomarkers as possible diagnostic tools for IR.
Two biomarker IR models combining levels of triglycerides (TG)/HDL, eotaxin, MCP-1 and PDGF-BB in pubertal patients with obesity of both sexes were defined. Altered levels of MCP-1, eotaxin, and PDGF-BB constitute a main component that determines 27.7% of the variance explaining IR. Growth and inflammatory factors comprise two other components linked to the first, together accounting for 59.2% of the variance determining IR.
PDGF-BB, MCP-1, eotaxin, TG and cholesterol concentrations constitute a solid panel of biomarkers associated with IR in pubertal children with obesity that could be useful in their stratification in a clinical setting for stratification.
肥胖症患者的脂肪组织会发生结构重塑，导致慢性低度炎症状态，并与胰岛素抵抗（IR）相关。
我们旨在开发一种与临床相关的生物标志物模型，用于对肥胖青少年进行 IR 分层。
对 143 名青春期肥胖患者（50% IR；50% 非 IR）和 33 名对照者的血清中细胞因子[肿瘤细胞衍生因子 1α、单核细胞趋化蛋白 1（MCP-1）、嗜酸性粒细胞趋化因子和 fractalkine]、生长因子[脑源性神经营养因子、促纤维化血小板衍生生长因子（PDGF-BB）和胰岛素样生长因子 1]和生化/代谢因子进行了分析。采用因子分析、相关性分析、二元逻辑回归和受试者工作特征分析，评估了这些生物标志物组合作为 IR 可能诊断工具的作用。
在两性肥胖的青春期患者中，定义了两个结合了甘油三酯（TG）/高密度脂蛋白（HDL）、嗜酸性粒细胞趋化因子、MCP-1 和 PDGF-BB 水平的生物标志物 IR 模型。MCP-1、嗜酸性粒细胞趋化因子和 PDGF-BB 的水平改变构成了决定 IR 的主要成分，决定了 27.7%的变异性。生长和炎症因子构成了与第一个因子相关的另外两个成分，共同决定了决定 IR 的 59.2%的变异性。
PDGF-BB、MCP-1、嗜酸性粒细胞趋化因子、TG 和胆固醇浓度构成了与肥胖青春期儿童 IR 相关的生物标志物的坚实组合，在临床环境中对其分层可能有用。
