Le Di, Pan Jie, Xing Haixia
Department of General Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China.
Materials (Basel). 2023 Sep 28;16(19):6450. doi: 10.3390/ma16196450.
To chemically functionalize the Ti6Al4V alloy surface, a custom-made low-temperature atmospheric pressure plasma reactor device was used to polymerize heptylamine on it. The effect of different deposition times, an important process parameter, was also investigated. For each deposition time group, the surface morphology was observed via scanning electron microscopy (SEM). The surface chemical content was analyzed via X-ray photoelectron spectroscopy, and surface hydrophilicity was measured via water contact angle. The adhesion of bone marrow stromal cells (BMSCs) on the modified Ti6Al4V alloy surfaces was also observed via SEM. A quantitative evaluation of cell proliferation was performed via the Cell Counting Kit-8 assay. The results revealed that amino groups were introduced on the Ti6Al4V alloy surface via plasma-polymerized heptylamine (PPHA). The percentages of NH/C for various deposition times (0 s, 30 s, 45 s, 60 s, 90 s, and 120 s) were 3.39%, 5.14%, 6.71%, 6.72%, 7.31%, and 7.65%. A 30 s, 45 s, and 60 s deposition time could significantly increase surface hydrophilicity with a mean water contact angle of 62.1 ± 1.6°, 65.7 ± 1.1°, and 88.2 ± 1.4°, respectively. Meanwhile, a 60 s, 90 s, and 120 s deposition time promoted BMSCs cell adhesion and proliferation. However, this promotion effect differed non-significantly among the three groups. In conclusion, the introduction of amino groups on the Ti6Al4V alloy surface exhibited surface modification and enhancement of cell adhesion and proliferation, which was partially associated with deposition time.
为了对Ti6Al4V合金表面进行化学功能化处理，使用定制的低温大气压等离子体反应器装置在其上聚合庚胺。还研究了重要工艺参数——不同沉积时间的影响。对于每个沉积时间组，通过扫描电子显微镜（SEM）观察表面形貌。通过X射线光电子能谱分析表面化学组成，并通过水接触角测量表面亲水性。还通过SEM观察骨髓基质细胞（BMSC）在改性Ti6Al4V合金表面上的粘附情况。通过细胞计数试剂盒-8检测对细胞增殖进行定量评估。结果表明，通过等离子体聚合庚胺（PPHA）在Ti6Al4V合金表面引入了氨基。不同沉积时间（0 s、30 s、45 s、60 s、90 s和120 s）下的NH/C百分比分别为3.39%、5.14%、6.71%、6.72%、7.31%和7.65%。沉积时间为30 s、45 s和60 s时可显著提高表面亲水性，平均水接触角分别为62.1±1.6°、65.7±1.1°和88.2±1.4°。同时，沉积时间为60 s、90 s和120 s时促进了BMSC细胞的粘附和增殖。然而，这三组之间的促进效果差异不显著。总之，在Ti6Al4V合金表面引入氨基表现出表面改性以及细胞粘附和增殖的增强，这部分与沉积时间有关。
