Lammi Carmen, Mulinacci Nadia, Cecchi Lorenzo, Bellumori Maria, Bollati Carlotta, Bartolomei Martina, Franchini Carlo, Clodoveo Maria Lisa, Corbo Filomena, Arnoldi Anna
Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy.
Department of Neuroscience, Psychology, Drug and Child Health, Pharmaceutical and Nutraceutical Section, University of Florence, 50019 Florence, Italy.
Antioxidants (Basel). 2020 Aug 27;9(9):798. doi: 10.3390/antiox9090798.
This study was aimed at demonstrating the substantial equivalence of two extra virgin olive oil samples extracted from the same batch of Coratina olives with (OMU) or without (OMN) using ultrasound technology, by performing chemical, biochemical, and cellular investigations. The volatile organic compounds compositions and phenolic profiles were very similar, showing that, while increasing the extraction yields, the innovative process does not change these features. The antioxidant and hypocholesterolemic activities of the extra virgin olive oil (EVOO) phenol extracts were also preserved, since OMU and OMN had equivalent abilities to scavenge the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals in vitro and to protect HepG2 cells from oxidative stress induced by HO, reducing intracellular reactive oxygen species (ROS) and lipid peroxidation levels. In addition, by inhibiting 3-hydroxy-3-methylglutarylcoenzyme a reductase, both samples modulated the low-density lipoprotein receptor (LDLR) pathway leading to increased LDLR protein levels and activity.
本研究旨在通过进行化学、生化和细胞研究，证明从同一批科拉蒂纳橄榄中提取的两份特级初榨橄榄油样品在使用（OMU）或不使用（OMN）超声技术的情况下具有实质等同性。挥发性有机化合物组成和酚类特征非常相似，表明在提高提取率的同时，创新工艺并未改变这些特征。特级初榨橄榄油（EVOO）酚提取物的抗氧化和降胆固醇活性也得以保留，因为OMU和OMN在体外清除1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基以及保护HepG2细胞免受HO诱导的氧化应激、降低细胞内活性氧（ROS）和脂质过氧化水平方面具有同等能力。此外，通过抑制3-羟基-3-甲基戊二酰辅酶A还原酶，两份样品均调节了低密度脂蛋白受体（LDLR）途径，导致LDLR蛋白水平和活性增加。
