Oliveira Tatiane Alexandre, Anágua-Bravo Ernesto, Aoki Idalina Vieira, Scaramucci Taís, Sobral Maria Angela Pita
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
Department of Chemical Engineering, Polytechnic School, University of São Paulo, São Paulo, SP, Brazil.
Microsc Res Tech. 2021 Feb;84(2):163-170. doi: 10.1002/jemt.23574. Epub 2020 Sep 1.
This study aimed to evaluate the capacity of novel experimental hybrid coatings (HC) to reduce dentin permeability and to verify their resistance to erosive and abrasive challenges. Dentin disc specimens (1 mm thick) were treated with 0.5 M EDTA solution and randomly allocated into three experimental groups (n = 10): Control (Saliva); Concentrated Hybrid Coating (TEOS/GPTMS/Y-APS); and Diluted Hybrid Coating (1:3 ratio with distilled water). Dentin permeability was assessed by hydraulic conductance in the following experimental time periods: post-EDTA, post treatment, post erosion (5 min in 0.05 M citric acid solution, pH = 3.8), and post abrasion (toothbrushing for 3,900 cycles). Dentin permeability percent was calculated with respect the values of post-EDTA for each experimental time. The morphology of the surface of extra dentin specimens was examined by scanning electron microscopy (SEM) in the same time periods (n = 3). Permeability data were analyzed by two-way repeated measures ANOVA and Tukey tests (p < .05). Both HC presented significantly lower dentin permeability than control post treatment and post erosion (p < .05), without difference between them (p > .05). Post abrasion, there were no significant difference among groups (p > .05). Post treatment and post erosion, the HC seemed to flow into the tubules, occluding them, while the tubules in control remained opened. Post abrasion, the tubules appear to be occluded in all groups. In conclusion, the experimental hybrid coatings were capable of reducing dentin permeability after treatment. They were also able to resist to erosive and abrasive challenges, with the advantage of forming thinner and colorless films that can be potentially used to treat dentin hypersensitivity.
本研究旨在评估新型实验性混合涂层（HC）降低牙本质渗透性的能力，并验证其对侵蚀和磨损挑战的抵抗力。将牙本质盘标本（1毫米厚）用0.5M乙二胺四乙酸（EDTA）溶液处理，并随机分为三个实验组（n = 10）：对照组（唾液）；浓缩混合涂层（正硅酸乙酯/γ-缩水甘油醚氧丙基三甲氧基硅烷/Y-氨丙基三乙氧基硅烷）；以及稀释混合涂层（与蒸馏水按1:3比例混合）。在以下实验时间段通过水力传导率评估牙本质渗透性：EDTA处理后、处理后、侵蚀后（在0.05M柠檬酸溶液中浸泡5分钟，pH = 3.8）和磨损后（刷牙3900次循环）。根据每个实验时间段EDTA处理后的数值计算牙本质渗透百分比。在相同时间段（n = 3）通过扫描电子显微镜（SEM）检查额外牙本质标本表面的形态。通过双向重复测量方差分析和Tukey检验分析渗透性数据（p < 0.05）。两种混合涂层在处理后和侵蚀后牙本质渗透性均显著低于对照组（p < 0.05），两者之间无差异（p > 0.05）。磨损后，各组之间无显著差异（p > 0.05）。处理后和侵蚀后，混合涂层似乎流入小管并将其堵塞，而对照组的小管仍保持开放。磨损后，所有组的小管似乎都被堵塞。总之，实验性混合涂层在处理后能够降低牙本质渗透性。它们还能够抵抗侵蚀和磨损挑战，其优点是形成更薄且无色的膜，可潜在用于治疗牙本质过敏症。
