Universidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, Brasil.
Indiana University School of Dentistry, Operative Dentistry and Dental Public Health, Department of Cariology, Indianapolis, United States.
J Appl Oral Sci. 2022 May 2;30:e20210643. doi: 10.1590/1678-7757-2021-0643. eCollection 2022.
The aim of this study is to test, in vitro, the anti-cariogenic effect of experimental hybrid coatings, with nano clays of halloysite or bentonite, loaded with sodium fluoride or with a combination of sodium fluoride and stannous chloride, respectively.
The varnish Fluor Protector (1,000 ppm of F-) was used as positive control and no treatment was the negative control. Enamel specimens (5 mm × 5 mm) were obtained from bovine teeth. The specimens (n=10) had their surfaces divided into two halves (5 mm × 2.5 mm each), in which one half received one of the treatments (Hybrid; Hybrid + NaF; Hybrid + NaF + SnCl2; Hybrid + NaF Loaded; Hybrid + NaF + SnCl2 Loaded). The specimens were submitted to a cariogenic challenge using a biofilm model (S. mutans UA159, for 5 days). Enamel surfaces both under and adjacent to the treated area were analyzed for mineral loss and lesion depth, by transverse microradiography. The pH of the medium was measured twice a day, and the fluoride release was analyzed. Additional specimens were submitted to confocal analysis.
Data were statistically analyzed by two-way ANOVA followed by Tukey test (α=0.05). None of hybrid groups were able to reduce the lesion depth; the Hybrid + NaF group, however, was able to reduce mineral loss differing from the negative control (p=0.008). The groups showed no significant difference in the pH measurement and fluoride release. Confocal analysis confirmed that for all groups the biofilm growth was similar.
None of the hybrid groups reduced lesion depth, but the Hybrid + NaF group was able to promote protection against mineral loss.
本研究旨在体外测试分别负载氟化物或氟化物与氯化亚锡的实验性杂化涂层的抗龋效果。
使用氟保护漆（1000ppmF-）作为阳性对照，不处理作为阴性对照。从牛牙获得釉质标本（5mm×5mm）。将标本（n=10）的表面分为两半（每半 5mm×2.5mm），其中一半接受一种处理（杂化；杂化+NaF；杂化+NaF+SnCl2；杂化+负载氟化物；杂化+负载氟化物+SnCl2）。使用生物膜模型（S.mutansUA159，持续 5 天）对标本进行致龋挑战。通过横向显微放射摄影术分析处理区域下方和相邻的釉质表面的矿物质损失和病变深度。每天测量两次介质的 pH 值，并分析氟化物释放情况。另外的标本进行共聚焦分析。
通过双因素方差分析和 Tukey 检验（α=0.05）对数据进行统计学分析。没有一个杂化组能够减少病变深度；然而，Hybrid+NaF 组能够减少矿物质损失，与阴性对照组相比差异有统计学意义（p=0.008）。在 pH 值测量和氟化物释放方面，各组之间无显著差异。共聚焦分析证实，对于所有组，生物膜生长相似。
没有一个杂化组能够减少病变深度，但 Hybrid+NaF 组能够促进对矿物质损失的保护。
