Faculdade São Leopoldo Mandic, Department of Restorative Dentistry and Dental Materials, Campinas, Brazil.
Acta Odontol Latinoam. 2022 Dec 31;35(3):206-213. doi: 10.54589/aol.35/3/206.
This study evaluated the influence of chitosan added to a universal adhesive system used in totaletch (TE) or self-etch (SE) mode on dentin permeability, and on the micromorphology of the adhesive layer.
Dentin discs were obtained from human third molars and randomly distributed according to bonding strategy (TE or SE), and to whether or not 1% chitosan (C) was added to a universal adhesive system (Single Bond Universal/3M ESPE), to create the following groups (n=10): TE, TEC, SE, and SEC. Dentin permeability was measured at baseline and after application of dentin treatments. The surface of the adhesive layer (AL) and the dentin adjacent to the AL were examined under a scanning electron microscope.
There were no significant differences in permeability percentage between the groups with and without C (TE and SE versus TEC and SEC) (p>0.05; Mann Whitney test). Dentin permeability was lower when the adhesive system was applied in the SE mode, regardless of the addition of C. The micromorphology of the AL surface showed irregularities, and a greater degree of porosity, when the adhesive system was applied in the SE mode, regardless of chitosan addition. There was a greater depth of penetration of the adhesives into the dentin adjacent to the AL in both the TE and TEC groups. Chitosan added to the adhesive system did not influence dentin permeability.
The self-etch strategy led to lower dentin permeability, and to more irregularities on the surface of the adhesive layer.
本研究评估了在全酸蚀（TE）或自酸蚀（SE）模式下，壳聚糖添加到通用粘结剂系统中对牙本质渗透性的影响，以及对粘结层微观形态的影响。
从人第三磨牙中获得牙本质圆盘，并根据粘结策略（TE 或 SE）以及是否向通用粘结剂系统（Single Bond Universal/3M ESPE）中添加 1%壳聚糖（C）随机分配，创建以下组（n=10）：TE、TEC、SE 和 SEC。在应用牙本质处理前后测量牙本质渗透性。在扫描电子显微镜下检查粘结层（AL）表面和紧邻 AL 的牙本质。
在有和没有 C 的组之间（TE 和 SE 与 TEC 和 SEC），渗透性百分比没有显著差异（p>0.05；Mann Whitney 检验）。无论是否添加 C，当粘结系统以 SE 模式应用时，牙本质渗透性较低。当粘结系统以 SE 模式应用时，无论是否添加壳聚糖，AL 表面的微观形态都显示出不规则性和更大程度的多孔性。在 TE 和 TEC 组中，粘结剂更深地渗透到紧邻 AL 的牙本质中。壳聚糖添加到粘结剂系统中不会影响牙本质渗透性。
自酸蚀策略导致牙本质渗透性降低，粘结层表面更不规则。
