Zhou Guocheng, Zhang Shaohui, Hu Yao, Hao Qun
Opt Lett. 2020 Sep 1;45(17):4956-4959. doi: 10.1364/OL.403995.
Fourier ptychography microscopy is a powerful tool for wide-field and high-spatial-resolution imaging. It can achieve a large field of view and high-spatial-resolution imaging with a low numerical aperture objective by capturing a series of low-resolution (LR) images that contain the information of different spatial frequencies and then stitching them together in the Fourier domain. Furthermore, the phase information of the object can also be recovered simultaneously. In this Letter, we propose a method to realize adaptive high-dynamic-range (HDR) LR image acquisition with a red, green, and blue camera, which needs only single exposure for each light-emitting diode (LED) illumination. According to the imaging principle of a color camera, the filter of each color channel still allows additional light within a certain wavelength range to pass through, but with a much smaller transmittance. By illuminating the sample with monochromatic light and combining the raw data from three color channels together, an HDR image corresponding to each LED illumination is obtained. The feasibility and good performance of our method are demonstrated by the experimental results.
傅里叶叠层显微镜是一种用于宽视场和高空间分辨率成像的强大工具。它可以通过捕获一系列包含不同空间频率信息的低分辨率（LR）图像，然后在傅里叶域中将它们拼接在一起，从而使用低数值孔径物镜实现大视场和高空间分辨率成像。此外，还可以同时恢复物体的相位信息。在本信函中，我们提出了一种使用红、绿、蓝相机实现自适应高动态范围（HDR）低分辨率图像采集的方法，该方法每个发光二极管（LED）照明仅需单次曝光。根据彩色相机的成像原理，每个颜色通道的滤光片仍允许一定波长范围内的额外光线通过，但透过率要小得多。通过用单色光照射样品并将来自三个颜色通道的原始数据组合在一起，可以获得与每个LED照明相对应的HDR图像。实验结果证明了我们方法的可行性和良好性能。
