Khairunisa Siti Qamariyah, Megasari Ni Luh Ayu, Rahayu Retno Pudji, Witaningrum Adiana Mutamsari, Ueda Shuhei, Yunifiar M Muhammad Qushai, Indriati Dwi Wahyu, Kotaki Tomohiro, Rusli Adria, Kameoka Masanori
Doctoral Program,Faculty of Medicine, Universitas Airlangga, Surabaya.
Indonesia-Japan Collaborative Research Centre for Emerging and Reemerging Infectious Diseases (CRCERI), Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
Infect Dis Rep. 2020 Jul 7;12(Suppl 1):8740. doi: 10.4081/idr.2020.8740.
The presence of transmitted drug resistance (TDR) in human immunodeficiency virus type 1 (HIV-1) infected individuals naive to antiretroviral therapy, may affect the effectiveness of treatment. Jakarta, the capital city of Indonesia, recorded the highest number of cumulative HIV infection cases in the country. This study aimed to identify on the appearance of TDR, as well as to identify HIV-1 subtypes circulating among treatment-naive individuals in Jakarta. Whole blood samples collected from 43 HIV-1 infected, treatment-naive individuals. Viral subtyping and drug resistance testing were performed on HIV-1 genes amplified using nested polymerase chain reaction. CRF01_AE was detected most frequently in Jakarta (73.08%). Drug resistance-related major mutation was not detected in protease fragments of gene, but two major mutations, K103N (6.67%) and Y181C (6.67%), were detected in reverse transcriptase fragments of gene. Our results suggest that TDR was emerged in Jakarta at a certain extent, thus further surveillance study to monitor the TDR prevalence and circulating HIV-1 subtypes in this region is considered to be necessary.
在未接受过抗逆转录病毒治疗的人类免疫缺陷病毒1型（HIV-1）感染者中存在传播性耐药（TDR），可能会影响治疗效果。印度尼西亚首都雅加达记录的该国累积HIV感染病例数最多。本研究旨在确定TDR的出现情况，以及确定雅加达未接受过治疗的个体中流行的HIV-1亚型。从43名未接受过治疗的HIV-1感染个体中采集全血样本。对使用巢式聚合酶链反应扩增的HIV-1基因进行病毒亚型分析和耐药性检测。在雅加达，CRF01_AE的检测频率最高（73.08%）。在该基因的蛋白酶片段中未检测到与耐药相关的主要突变，但在该基因的逆转录酶片段中检测到两个主要突变，K103N（6.67%）和Y181C（6.67%）。我们的结果表明，TDR在雅加达已在一定程度上出现，因此认为有必要进一步开展监测研究，以监测该地区TDR的流行情况和流行的HIV-1亚型。
