Khairunisa Siti Qamariyah, Ueda Shuhei, Witaningrum Adiana Mutamsari, Yunifiar Matondang Muhammad Qushai, Indriati Dwi Wahyu, Kotaki Tomohiro, Kameoka Masanori
1 Indonesian-Japan Collaborative Research Center for Emerging and Re-Emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University , Surabaya, Indonesia .
2 Center for Infectious Diseases, Kobe University Graduate School of Medicine , Hyogo, Japan .
AIDS Res Hum Retroviruses. 2018 Jun;34(6):555-560. doi: 10.1089/AID.2018.0040. Epub 2018 Apr 24.
Kepulauan Riau is a famous tourist destination in Indonesia. The epidemic of human immunodeficiency virus type 1 (HIV-1) infection is gradually increasing in this region. We collected peripheral blood samples from 62 antiretroviral therapy-experienced individuals. The amplification of viral genomic fragments, HIV-1 subtyping, and the detection of HIV drug resistance (HIVDR) were performed. Viral subtyping revealed that the most prevalent HIV-1 subtype/circulating recombinant form (CRF) was CRF01_AE (55.6%), followed by recombinants between CRF01_AE and subtype B (17.8%) and then subtype B (15.6%). Recombinants containing CRF02_AG gene fragments were also detected (11.1%). Regarding HIVDR, no drug resistance-associated major mutations were found in pol genes encoding protease, although minor mutations were frequently detected. Furthermore, major mutations, including M184V (2.2%) and Y188L (2.2%), were identified in the viral pol gene encoding reverse transcriptase derived from a study participant, suggesting that the prevalence of HIVDR is low in the region.
廖内群岛是印度尼西亚一个著名的旅游胜地。1型人类免疫缺陷病毒（HIV-1）感染疫情在该地区正逐渐增加。我们从62名有抗逆转录病毒治疗经验的个体中采集了外周血样本。进行了病毒基因组片段扩增、HIV-1亚型分析以及HIV耐药性（HIVDR）检测。病毒亚型分析显示，最常见的HIV-1亚型/循环重组型（CRF）是CRF01_AE（55.6%），其次是CRF01_AE与B亚型之间的重组型（17.8%），然后是B亚型（15.6%）。还检测到含有CRF02_AG基因片段的重组型（11.1%）。关于HIVDR，在编码蛋白酶的pol基因中未发现与耐药相关的主要突变，尽管经常检测到 minor 突变。此外，在一名研究参与者来源的编码逆转录酶的病毒pol基因中鉴定出了包括M184V（2.2%）和Y188L（2.2%）在内的主要突变，这表明该地区HIVDR的流行率较低。
