Izutani-Kitano Ai, Okada Yuka, Ichikawa Kana, Reinach Peter Sol, Saika Shizuya
Department of Ophthalmology, Kihoku Hospital, Wakayama Medical University, Wakayama, Japan.
Department of Ophthalmology, School of Medicine, Wakayama Medical University, Wakayama, Japan.
Taiwan J Ophthalmol. 2020 May 19;10(2):106-110. doi: 10.4103/tjo.tjo_12_20. eCollection 2020 Apr-Jun.
We determined if the immunohistochemical expression pattern of transient receptor potential vanilloid (TRPV) family members and TRP ankyrin 1 (TRPA1) differs among a healthy conjunctival epithelium and diseased epithelia.
Subjects include a normal conjunctival epithelium, pterygium epithelium, epithelial dysplasia or carcinoma .
TRPV1, TRPV4 or TRPA1 was detected in both the cytoplasm and nuclei, or in either the nuclei or cytoplasm, of these different epithelial layers, respectively. There was no difference in the expression pattern of these three TRP isoforms. On the other hand, the expression patterns of TRPV2 and TRPV3 differed dramatically among these different subjects. TRPV2 was observed in the basal layer epithelium of a normal conjunctiva and pterygium. Its pattern was scattered in this region, although TRPV2 was absent throughout most of the dysplastic epithelium. TRPV2 was detected only in some of the suprabasal epithelial cells of a carcinoma . TRPV3 was faintly detected in the cytoplasm of all the cell layers and also in the nuclei of some of the basal cells in a normal conjunctiva and in the pterygia epithelium, while it was uniformly expressed in all of the dysplasia and carcinoma nuclei in all epithelial cell layers.
These results suggest that TRPV2 and TRPV3 expression pattern analysis might be potential diagnostic markers of ocular surface epithelial disorders.
我们确定了瞬时受体电位香草酸亚家族（TRPV）成员和锚蛋白1型瞬时受体电位（TRPA1）在健康结膜上皮和病变上皮中的免疫组化表达模式是否存在差异。
研究对象包括正常结膜上皮、翼状胬肉上皮、上皮发育异常或癌组织。
TRPV1、TRPV4或TRPA1分别在这些不同上皮层的细胞质和细胞核中，或仅在细胞核或细胞质中被检测到。这三种TRP亚型的表达模式没有差异。另一方面，TRPV2和TRPV3的表达模式在这些不同的研究对象中存在显著差异。TRPV2在正常结膜和翼状胬肉的基底层上皮中被观察到。其模式在该区域呈散在分布，尽管在大多数发育异常的上皮中未检测到TRPV2。TRPV2仅在癌组织的一些基底上层上皮细胞中被检测到。TRPV3在正常结膜和翼状胬肉上皮的所有细胞层的细胞质中以及一些基底细胞的细胞核中被微弱检测到，而在所有上皮细胞层的发育异常和癌组织细胞核中均呈均匀表达。
这些结果表明，TRPV2和TRPV3表达模式分析可能是眼表上皮疾病的潜在诊断标志物。
