Cell Biology Department, Instituto Nacional de Perinatologia 'Isidro Espinosa de los Reyes', Mexico City, Mexico.
Am J Reprod Immunol. 2012 Jan;67(1):34-43. doi: 10.1111/j.1600-0897.2011.01054.x. Epub 2011 Jul 13.
Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis.
Using a two-compartment experimental model, 1 × 10(6) CFU/mL of G. vaginalis were added to either the amnion or choriodecidua face or to both. Concentrations of IL-1β, TNF-α, and IL-6, as well as human beta defensins (HBD) 1-3 were quantified by ELISA.
In comparison with control conditions and regardless of the stimulation modality, IL-1β and IL-6 increased 4-fold and 28-fold, respectively, in the choriodecidual compartment. HBD-1 increased 2-fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD-2 and HBD-3 increased an average of 2- and 8-fold, respectively, in the choriodecidual region.
Stimulation with G. vaginalis induced a tissue-specific secretion profile of 1L-1β, IL-6, and HBD 1-3 in the chorioamniotic membranes.
与感染相关的早产是一种主要的临床情况；在这项工作中，我们分析了人羊膜绒毛膜被阴道加德纳菌刺激后的反应。
使用两室实验模型，将 1×10(6)CFU/mL 的阴道加德纳菌添加到羊膜或绒毛膜蜕膜的任一面或两面。通过 ELISA 定量测定 IL-1β、TNF-α 和 IL-6 以及人β防御素 (HBD)1-3 的浓度。
与对照条件相比，无论刺激方式如何，在绒毛膜蜕膜腔中，IL-1β 和 IL-6 分别增加了 4 倍和 28 倍。当刺激物直接作用于该区域时，HBD-1 在羊膜腔中增加了 2 倍。HBD-2 和 HBD-3 在绒毛膜蜕膜区分别平均增加了 2 倍和 8 倍。
阴道加德纳菌的刺激在羊膜绒毛膜中诱导了组织特异性的 IL-1β、IL-6 和 HBD 1-3 分泌谱。
