Saxena Somil, Venugopal Ranganath, Chandrayan Rao Rachana, Yuwanati Monal B, Awasthi Harshita, Jain Megha
People's College of Dental Sciences and Research Centre, People's University, Bhopal, India.
AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, India.
J Oral Biol Craniofac Res. 2020 Oct-Dec;10(4):529-534. doi: 10.1016/j.jobcr.2020.08.013. Epub 2020 Aug 19.
Chronic periodontitis (CP) and Type 2 diabetes mellitus (T2DM) are chronic diseases affecting the humans and have interrelationship in their pathogenesis. Monitoring the progress with biomarker in these disease is important from treatment outcome point of view. We investigated the association of salivary developmental endothelial locus-1 (Del-1) and interleukin-17 (IL-17) levels in CP and T2DM.
Saliva was collected from 17 healthy and 68 patients (divided into 4 groups based on presence or absence of CP and T2DM). Periodontal parameters were recorded at the time of visit. Del-1 and IL-17 levels in unstimulated salivary samples were determined using enzyme-linked immunosorbent assay.
Study found the lower levels of salivary Del-1 and higher levels of IL-17 in CP (2.23 ± 2.10 ng/mL, 56.54 ± 19.79 ng/mL), CCDM ((1.97 ± 1.36 ng/mL, 74.74 ± 17.79 ng/mL) and CUDM (1.02 ± 0.52 ng/mL, 63.84 ± 24.72 ng/mL) as compared to healthy individuals (6.54 ± 2.07; 24.47 ± 8.23 ng/mL) (p < 0.001). Del-1 and IL-17 levels were correlating with inflammatory state in CP and presence of T2DM aggravate the severity of disease in CP. An inverse correlation between salivary Del-1 & IL-17 levels was also seen. Furthermore, we also observed that the combination of T2DM and CP (CCDM and CUDM) increases the salivary IL-17 levels and decreases Del-1 levels.
There is an upregulation of salivary IL-17 and downregulation of salivary Del-1 with increase in severity of periodontal disease as well as T2DM. Furthermore, the presence of T2DM in chronic periodontitis patients can aggravate the inflammation related periodontal destruction.
慢性牙周炎（CP）和2型糖尿病（T2DM）是影响人类的慢性疾病，其发病机制存在相互关系。从治疗结果的角度来看，用生物标志物监测这些疾病的进展很重要。我们研究了慢性牙周炎和2型糖尿病患者唾液中发育性内皮位点-1（Del-1）和白细胞介素-17（IL-17）水平的相关性。
收集了17名健康人和68名患者的唾液（根据是否存在慢性牙周炎和2型糖尿病分为4组）。在就诊时记录牙周参数。使用酶联免疫吸附测定法测定未刺激唾液样本中的Del-1和IL-17水平。
研究发现，与健康个体（6.54±2.07；24.47±8.23 ng/mL）相比，慢性牙周炎组（2.23±2.10 ng/mL，56.54±19.79 ng/mL）、慢性牙周炎合并2型糖尿病组（CCDM，1.97±1.36 ng/mL，74.74±17.79 ng/mL）和2型糖尿病合并慢性牙周炎组（CUDM，1.02±0.52 ng/mL，63.
