Association of chronic periodontitis and type 2 diabetes mellitus with salivary Del-1 and IL-17 levels.

PURPOSE

Chronic periodontitis (CP) and Type 2 diabetes mellitus (T2DM) are chronic diseases affecting the humans and have interrelationship in their pathogenesis. Monitoring the progress with biomarker in these disease is important from treatment outcome point of view. We investigated the association of salivary developmental endothelial locus-1 (Del-1) and interleukin-17 (IL-17) levels in CP and T2DM.

METHODS

Saliva was collected from 17 healthy and 68 patients (divided into 4 groups based on presence or absence of CP and T2DM). Periodontal parameters were recorded at the time of visit. Del-1 and IL-17 levels in unstimulated salivary samples were determined using enzyme-linked immunosorbent assay.

RESULTS

Study found the lower levels of salivary Del-1 and higher levels of IL-17 in CP (2.23 ± 2.10 ng/mL, 56.54 ± 19.79 ng/mL), CCDM ((1.97 ± 1.36 ng/mL, 74.74 ± 17.79 ng/mL) and CUDM (1.02 ± 0.52 ng/mL, 63.84 ± 24.72 ng/mL) as compared to healthy individuals (6.54 ± 2.07; 24.47 ± 8.23 ng/mL) (p < 0.001). Del-1 and IL-17 levels were correlating with inflammatory state in CP and presence of T2DM aggravate the severity of disease in CP. An inverse correlation between salivary Del-1 & IL-17 levels was also seen. Furthermore, we also observed that the combination of T2DM and CP (CCDM and CUDM) increases the salivary IL-17 levels and decreases Del-1 levels.

CONCLUSIONS

There is an upregulation of salivary IL-17 and downregulation of salivary Del-1 with increase in severity of periodontal disease as well as T2DM. Furthermore, the presence of T2DM in chronic periodontitis patients can aggravate the inflammation related periodontal destruction.