Instituto de Quı́mica (IQ), Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6154, CEP 13083-970 Campinas, São Paulo, Brasil.
Pós Graduação em Biotecnociência, Universidade Federal do ABC, CEP 09210-580 Santo André, Brasil.
Langmuir. 2020 Oct 6;36(39):11442-11449. doi: 10.1021/acs.langmuir.0c01571. Epub 2020 Sep 22.
The outreach of nanoparticle-based medical treatments has been severely hampered due to the imbalance between the efforts in designing extremely complex materials and the general lack of studies devoted to understanding their colloidal stability in biological environments. Over the years, the scientific community has neglected the relevance related to the nanoparticles' colloidal state, which consequently resulted in very poor bench-to-clinic translation. In this work, we show how mesoporous silica nanoparticles (MSNs, one of the most promising and tested drug delivery platforms) can be efficiently synthesized and prepared, resulting in a colloidally stable system. We first compared three distinct methods of template removal of MSNs and evaluated their ultimate colloidal stability. Then, we also proposed a simple way to prevent aggregation during the drying step by adsorbing BSA onto MSNs. The surface modification resulted in colloidally stable particles that are successfully redispersed in biologically relevant medium while retaining high hemocompatibility and low cytotoxicity.
由于在设计极其复杂的材料方面所付出的努力与致力于了解其在生物环境中的胶体稳定性的研究之间的不平衡，基于纳米粒子的医疗方法的推广受到了严重阻碍。多年来，科学界一直忽略了与纳米粒子胶体状态相关的重要性，这导致了从实验室到临床的转化非常不理想。在这项工作中，我们展示了如何高效地合成和制备介孔硅纳米粒子（MSNs，最有前途和经过充分测试的药物输送平台之一），从而得到胶体稳定的体系。我们首先比较了三种不同的 MSNs 模板去除方法，并评估了它们的最终胶体稳定性。然后，我们还提出了一种通过将 BSA 吸附到 MSNs 上来防止在干燥步骤中聚集的简单方法。表面改性得到了胶体稳定的颗粒，这些颗粒可以在生物相关介质中成功重新分散，同时保持高血液相容性和低细胞毒性。
