Tiagunenko Iu V, Petkov A S, Kirova N Z, Avramova R S, Leseva M D
Antibiot Khimioter. 1988 Jan;33(1):46-51.
Apramycin-modifying strains isolated from pigs with coli bacteriosis, from humans and hospital environment were studied comparatively. Production of enzymes modifying the aminoglycoside was estimated with the radioactive cofactor procedure. E. coli isolates from the animals were phenotypically resistant to apramycin and a number of other aminoglycosides. They produced acetyltransferase AAC(3)IV, phosphotransferase APH(3')(5"), APH(3") and other enzymes. Resistance of the strains to gentamicin was also conditioned by AAC(3)IV since these strains did not produce AAD(2") and AAC(6'). In the resistant strains of E. coli and their transconjugates there were detected plasmids with a relative molecular weight of 60-80 MD. Some of the belonged to the compatibility group I1, the others belonged to the compatibility group H1. Strains of S. marcescens, K. pneumoniae. K. oxytoca and S. aureus isolated from humans and hospital environment were sensitive to apramycin. Only isolates of P. aeruginosa were resistant to this antibiotic. However, all the isolates produced AAC(3)IV. Some of them additionally produced AAC(6'), an enzyme modifying amikacin, kanamycin and other antibiotics and not acetylating apramycin. Almost all the strains produced kanamycin- and streptomycin phosphotransferases. Possible coselection of strains resistant to apramycin and gentamicin using one of these aminoglycosides is discussed.
对从患有大肠杆菌病的猪、人类以及医院环境中分离出的阿普拉霉素修饰菌株进行了比较研究。采用放射性辅因子法评估修饰氨基糖苷类的酶的产生情况。从动物中分离出的大肠杆菌菌株在表型上对阿普拉霉素和其他多种氨基糖苷类耐药。它们产生乙酰转移酶AAC(3)IV、磷酸转移酶APH(3')(5")、APH(3")及其他酶。这些菌株对庆大霉素的耐药性也由AAC(3)IV决定，因为它们不产生AAD(2")和AAC(6')。在大肠杆菌的耐药菌株及其转接合子中检测到相对分子质量为60 - 80 MD的质粒。其中一些属于相容群I1，另一些属于相容群H1。从人类和医院环境中分离出的粘质沙雷氏菌、肺炎克雷伯菌、产酸克雷伯菌和金黄色葡萄球菌菌株对阿普拉霉素敏感。只有铜绿假单胞菌的分离株对这种抗生素耐药。然而，所有分离株都产生AAC(3)IV。其中一些还产生AAC(6')，这是一种修饰阿米卡星、卡那霉素和其他抗生素但不使阿普拉霉素乙酰化的酶。几乎所有菌株都产生卡那霉素和链霉素磷酸转移酶。讨论了使用其中一种氨基糖苷类对阿普拉霉素和庆大霉素耐药菌株进行共选择的可能性。
