Chiaino Elda, Micucci Matteo, Cosconati Sandro, Novellino Ettore, Budriesi Roberta, Chiarini Alberto, Frosini Maria
Dipartimento di Scienze della Vita, Università di Siena, Via Aldo Moro, 2, 53100 Siena, Italy.
Dipartimento di Farmacia e Biotecnologie, Alma Mater Studiorum, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Antioxidants (Basel). 2020 Sep 1;9(9):806. doi: 10.3390/antiox9090806.
Oxidative stress (OS) arising from tissue redox imbalance, critically contributes to the development of neurodegenerative disorders. Thus, natural compounds, owing to their antioxidant properties, have promising therapeutic potential. (PRES) is a nutraceutical product composed of leaves- and flowers-extracts of L. and L., respectively, the composition of which has been characterized by HPLC coupled to a UV-Vis and QqQ-Ms detector. As PRES possess antioxidant, antiapoptotic and anti-inflammatory properties, the aim of this study was to assess its neuroprotective effects in human neuroblastoma SH-SY5Y cells and in rat brain slices subjected to OS. PRES (1-50 µg/mL) reverted the decrease in viability as well as the increase in sub-diploid-, DAPI-and annexin V-positive-cells, reduced ROS formation, recovered the mitochondrial potential and caspase-3 and 9 activity changes caused by OS. PRES (50-100 µg/mL) neuroprotective effects occurred also in rat brain slices subjected to HO challenge. Finally, as the neuroprotective potential of PRES is strictly related to its penetration into the brain and a relatively good pharmacokinetic profile, an in-silico prediction of its components drug-like properties was carried out. The present results suggest the possibility of PRES as a nutraceutical, which could help in preventing neurodegenerative diseases.
组织氧化还原失衡引起的氧化应激（OS）对神经退行性疾病的发展起着关键作用。因此，天然化合物因其抗氧化特性具有广阔的治疗潜力。（PRES）是一种营养保健品，分别由L.和L.的叶提取物和花提取物组成，其成分已通过与紫外可见和三重四极杆质谱检测器联用的高效液相色谱法进行了表征。由于PRES具有抗氧化、抗凋亡和抗炎特性，本研究的目的是评估其在人神经母细胞瘤SH-SY5Y细胞和遭受氧化应激的大鼠脑片中的神经保护作用。PRES（1-50μg/mL）逆转了细胞活力的下降以及亚二倍体、DAPI和膜联蛋白V阳性细胞的增加，减少了活性氧的形成，恢复了线粒体电位以及由氧化应激引起的半胱天冬酶-3和-9活性变化。PRES（50-100μg/mL）在遭受HO攻击的大鼠脑片中也具有神经保护作用。最后，由于PRES的神经保护潜力与其对大脑的渗透以及相对良好的药代动力学特征密切相关，因此对其成分的类药物性质进行了计算机模拟预测。目前的结果表明PRES作为一种营养保健品有助于预防神经退行性疾病的可能性。
