• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA SNHG6通过吸附miR-485-3p增强宫颈癌细胞的放射抗性并促进其生长。

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p.

作者信息

Liu Jin, Liu Xiaojiao, Li Rong

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an, 710061 Shaanxi China.

Department of Mammary Gland & Thyroid Surgery, Sichuan Gem Flower Hospital, No. 26 Tongjixiang Road, Chengdu, 610213 Sichuan China.

出版信息

Cancer Cell Int. 2020 Aug 31;20:424. doi: 10.1186/s12935-020-01448-9. eCollection 2020.

DOI:10.1186/s12935-020-01448-9
PMID:32884447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7457785/
Abstract

BACKGROUND

Cervical cancer (CC) is the one of most common malignant gynecological tumors, which is characterized with the high mortality and recurrence rate. Previous studies have elucidated the oncogenic role of small nucleolar RNA host gene 6 (SNHG6) in some types of human cancers, whereas it is unclear whether it functions as an oncogene in CC. This study was aimed at unveiling the role of SNHG6 in CC.

METHODS

qRT-PCR analysis was implemented to evaluate the expression levels of SNHG6, miR-485-3p and STYX in CC cells. RNA pull down assay and luciferase reporter assay were conducted to verify the interaction between miR-485-3p and SNHG6 or STYX. Functional assays, such as colony formation assay, JC-1 assay and TUNEL assay were applied to detect the biological behaviors of CC cells. The resistance of CC cells to radiation was evaluated by colony formation assay.

RESULTS

SNHG6 was expressed at a high level in CC cells. Silenced SNHG6 suppressed cell proliferation but promoted cell apoptosis. Additionally, silenced SNHG6 could sensitize CC cells to radiation treatment. miR-485-3p could bind to both SNHG6 and STYX. Knockdown of miR-485-3p or overexpression of STYX could abolish the effects of SNHG6 silencing on CC cell growth.

CONCLUSIONS

LncRNA SNHG6 enhances the radioresistance of CC cells and promotes CC cell growth by sponging miR-485-3p to release STYX.

摘要

背景

宫颈癌（CC）是最常见的妇科恶性肿瘤之一，其特点是死亡率和复发率高。先前的研究已经阐明了小核仁RNA宿主基因6（SNHG6）在某些类型的人类癌症中的致癌作用，然而其在宫颈癌中是否作为癌基因发挥作用尚不清楚。本研究旨在揭示SNHG6在宫颈癌中的作用。

方法

采用qRT-PCR分析评估SNHG6、miR-485-3p和STYX在宫颈癌细胞中的表达水平。进行RNA下拉试验和荧光素酶报告试验以验证miR-485-3p与SNHG6或STYX之间的相互作用。应用集落形成试验、JC-1试验和TUNEL试验等功能试验来检测宫颈癌细胞的生物学行为。通过集落形成试验评估宫颈癌细胞对辐射的抗性。

结果

SNHG6在宫颈癌细胞中高表达。沉默SNHG6可抑制细胞增殖但促进细胞凋亡。此外，沉默SNHG6可使宫颈癌细胞对放射治疗敏感。miR-485-3p可与SNHG6和STYX结合。敲低miR-485-3p或过表达STYX可消除SNHG6沉默对宫颈癌细胞生长的影响。

结论

长链非编码RNA SNHG6通过海绵吸附miR-485-3p释放STYX来增强宫颈癌细胞的放射抗性并促进宫颈癌细胞生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/8ecbfeee10d7/12935_2020_1448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/67ccf1d65d62/12935_2020_1448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/246bc4dd466c/12935_2020_1448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/fc56d772155c/12935_2020_1448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/8ecbfeee10d7/12935_2020_1448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/67ccf1d65d62/12935_2020_1448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/246bc4dd466c/12935_2020_1448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/fc56d772155c/12935_2020_1448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2814/7457785/8ecbfeee10d7/12935_2020_1448_Fig4_HTML.jpg

相似文献

1
LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p.长链非编码RNA SNHG6通过吸附miR-485-3p增强宫颈癌细胞的放射抗性并促进其生长。
Cancer Cell Int. 2020 Aug 31;20:424. doi: 10.1186/s12935-020-01448-9. eCollection 2020.
2
LncRNA SNHG6 is Associated with Poor Prognosis of Gastric Cancer and Promotes Cell Proliferation and EMT through Epigenetically Silencing p27 and Sponging miR-101-3p.长链非编码RNA SNHG6与胃癌的不良预后相关，并通过表观遗传沉默p27和海绵化miR-101-3p促进细胞增殖和上皮-间质转化。
Cell Physiol Biochem. 2017;42(3):999-1012. doi: 10.1159/000478682. Epub 2017 Jun 27.
3
Long Noncoding RNA SNHG6 Promotes Proliferation and Inhibits Apoptosis in Non-small Cell Lung Cancer Cells by Regulating miR-490-3p/RSF1 Axis.长链非编码 RNA SNHG6 通过调控 miR-490-3p/RSF1 轴促进非小细胞肺癌细胞的增殖并抑制其凋亡。
Cancer Biother Radiopharm. 2020 Jun;35(5):351-361. doi: 10.1089/cbr.2019.3120. Epub 2020 Mar 23.
4
LncRNA SNHG6 Inhibits Apoptosis by Regulating EZH2 Expression via the Sponging of MiR-101-3p in Esophageal Squamous-Cell Carcinoma.长链非编码RNA SNHG6通过在食管鳞状细胞癌中吸附miR-101-3p来调节EZH2表达从而抑制细胞凋亡。
Onco Targets Ther. 2020 Nov 6;13:11411-11420. doi: 10.2147/OTT.S275135. eCollection 2020.
5
Long non-coding RNA SNHG6 promotes glioma tumorigenesis by sponging miR-101-3p.长链非编码RNA SNHG6通过吸附miR-101-3p促进胶质瘤的肿瘤发生。
Int J Biol Markers. 2018 May;33(2):148-155. doi: 10.1177/1724600817747524.
6
Long non-coding RNA SNHG6 promotes tumorigenesis in melanoma cells via the microRNA-101-3p/RAP2B axis.长链非编码RNA SNHG6通过微小RNA-101-3p/RAP2B轴促进黑色素瘤细胞的肿瘤发生。
Oncol Lett. 2020 Dec;20(6):323. doi: 10.3892/ol.2020.12186. Epub 2020 Oct 5.
7
Long non-coding RNA SNHG6 promotes the growth and invasion of non-small cell lung cancer by downregulating miR-101-3p.长链非编码 RNA SNHG6 通过下调 miR-101-3p 促进非小细胞肺癌的生长和侵袭。
Thorac Cancer. 2020 May;11(5):1180-1190. doi: 10.1111/1759-7714.13371. Epub 2020 Mar 9.
8
SNHG6 Interacted with miR-325-3p to Regulate Cisplatin Resistance of Gastric Cancer by Targeting GITR.SNHG6通过靶向GITR与miR-325-3p相互作用来调节胃癌的顺铂耐药性。
Onco Targets Ther. 2020 Nov 25;13:12181-12193. doi: 10.2147/OTT.S262896. eCollection 2020.
9
SNHG 6 promotes the progression of Colon and Rectal adenocarcinoma via miR-101-3p and Wnt/β-catenin signaling pathway.SNHG6 通过 miR-101-3p 和 Wnt/β-catenin 信号通路促进结直肠腺癌的进展。
BMC Gastroenterol. 2019 Sep 18;19(1):163. doi: 10.1186/s12876-019-1080-3.
10
Long noncoding RNA SNHG6 promotes oesophageal squamous cell carcinoma by downregulating the miR-101-3p/EZH2 pathway.长链非编码 RNA SNHG6 通过下调 miR-101-3p/EZH2 通路促进食管鳞状细胞癌。
J Biochem Mol Toxicol. 2022 Feb;36(2):e22959. doi: 10.1002/jbt.22959. Epub 2021 Nov 12.

引用本文的文献

1
The research progress on radiation resistance of cervical cancer.宫颈癌放射抗性的研究进展
Front Oncol. 2024 Apr 8;14:1380448. doi: 10.3389/fonc.2024.1380448. eCollection 2024.
2
LncRNA SNHG6 role in clinicopathological parameters in cancers.长链非编码 RNA SNHG6 在癌症临床病理参数中的作用。
Eur J Med Res. 2023 Sep 21;28(1):363. doi: 10.1186/s40001-023-01358-2.
3
Review of possible mechanisms of radiotherapy resistance in cervical cancer.宫颈癌放疗抵抗的可能机制综述。

本文引用的文献

1
SNHG 6 promotes the progression of Colon and Rectal adenocarcinoma via miR-101-3p and Wnt/β-catenin signaling pathway.SNHG6 通过 miR-101-3p 和 Wnt/β-catenin 信号通路促进结直肠腺癌的进展。
BMC Gastroenterol. 2019 Sep 18;19(1):163. doi: 10.1186/s12876-019-1080-3.
2
Downregulation of miR-485-3p promotes glioblastoma cell proliferation and migration via targeting RNF135.miR-485-3p的下调通过靶向RNF135促进胶质母细胞瘤细胞的增殖和迁移。
Exp Ther Med. 2019 Jul;18(1):475-482. doi: 10.3892/etm.2019.7600. Epub 2019 May 23.
3
LncRNA SNHG10 Facilitates Hepatocarcinogenesis and Metastasis by Modulating Its Homolog SCARNA13 via a Positive Feedback Loop.
Front Oncol. 2023 Aug 22;13:1164985. doi: 10.3389/fonc.2023.1164985. eCollection 2023.
4
Molecular mechanisms of long noncoding RNAs associated with cervical cancer radiosensitivity.与宫颈癌放射敏感性相关的长链非编码RNA的分子机制
Front Genet. 2023 Jan 4;13:1093549. doi: 10.3389/fgene.2022.1093549. eCollection 2022.
5
Cervical cancer heterogeneity: a constant battle against viruses and drugs.宫颈癌异质性：与病毒和药物的持续斗争。
Biomark Res. 2022 Nov 17;10(1):85. doi: 10.1186/s40364-022-00428-7.
6
LncRNA SNHG6 sponges miR-101 and induces tamoxifen resistance in breast cancer cells through induction of EMT.长链非编码RNA SNHG6作为微小RNA-101的海绵，通过诱导上皮-间质转化诱导乳腺癌细胞对他莫昔芬耐药。
Front Oncol. 2022 Sep 23;12:1015428. doi: 10.3389/fonc.2022.1015428. eCollection 2022.
7
LncRNA SNHG6 inhibits autophagy of gastric carcinoma cells via PI3K/AKT/mTOR signaling pathway.长链非编码RNA SNHG6通过PI3K/AKT/mTOR信号通路抑制胃癌细胞的自噬。
Am J Transl Res. 2022 May 15;14(5):2861-2873. eCollection 2022.
8
The Role of LncRNAs in the Regulation of Radiotherapy Sensitivity in Cervical Cancer.长链非编码RNA在宫颈癌放疗敏感性调控中的作用
Front Oncol. 2022 May 26;12:896840. doi: 10.3389/fonc.2022.896840. eCollection 2022.
9
Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284.长链非编码 RNA PGM5-AS1 通过海绵吸附 miR-4284 上调 Decorin（DCN）抑制宫颈癌进展。
Bioengineered. 2022 Apr;13(4):9872-9884. doi: 10.1080/21655979.2022.2062088.
10
The long and short non-coding RNAs modulating EZH2 signaling in cancer.长链和短链非编码 RNA 调节癌症中的 EZH2 信号通路。
J Hematol Oncol. 2022 Mar 2;15(1):18. doi: 10.1186/s13045-022-01235-1.
长链非编码 RNA SNHG10 通过正反馈环路调节其同源物 SCARNA13 促进肝癌发生和转移。
Cancer Res. 2019 Jul 1;79(13):3220-3234. doi: 10.1158/0008-5472.CAN-18-4044. Epub 2019 May 17.
4
Pseudophosphatase STYX promotes tumor growth and metastasis by inhibiting FBXW7 function in colorectal cancer.假磷酸酶 STYX 通过抑制结直肠癌中 FBXW7 的功能促进肿瘤生长和转移。
Cancer Lett. 2019 Jul 10;454:53-65. doi: 10.1016/j.canlet.2019.04.014. Epub 2019 Apr 11.
5
LncRNA DANCR promotes cervical cancer progression by upregulating ROCK1 via sponging miR-335-5p.长链非编码 RNA DANCR 通过海绵吸附 miR-335-5p 上调 ROCK1 促进宫颈癌进展。
J Cell Physiol. 2019 May;234(5):7266-7278. doi: 10.1002/jcp.27484. Epub 2018 Oct 26.
6
SNHG6 functions as a competing endogenous RNA to regulate E2F7 expression by sponging miR-26a-5p in lung adenocarcinoma.SNHG6 通过海绵吸附 miR-26a-5p 作为竞争内源性 RNA 调控肺腺癌中 E2F7 的表达。
Biomed Pharmacother. 2018 Nov;107:1434-1446. doi: 10.1016/j.biopha.2018.08.099. Epub 2018 Sep 1.
7
SNHG6 Promotes Tumor Growth via Repression of P21 in Colorectal Cancer.SNHG6通过抑制结直肠癌中的P21促进肿瘤生长。
Cell Physiol Biochem. 2018;49(2):463-478. doi: 10.1159/000492986. Epub 2018 Aug 29.
8
LncRNA NEAT1 regulates cervical carcinoma proliferation and invasion by targeting AKT/PI3K.长链非编码 RNA NEAT1 通过靶向 AKT/PI3K 调节宫颈癌的增殖和侵袭。
Eur Rev Med Pharmacol Sci. 2018 Jul;22(13):4090-4097. doi: 10.26355/eurrev_201807_15400.
9
LncRNA SNHG7 sponges miR-216b to promote proliferation and liver metastasis of colorectal cancer through upregulating GALNT1.LncRNA SNHG7 通过海绵吸附 miR-216b 促进结直肠癌细胞的增殖和肝转移，上调 GALNT1 表达。
Cell Death Dis. 2018 Jun 18;9(7):722. doi: 10.1038/s41419-018-0759-7.
10
LncRNA SNHG1 regulates the differentiation of Treg cells and affects the immune escape of breast cancer via regulating miR-448/IDO.长链非编码 RNA SNHG1 通过调节 miR-448/IDO 调控 Treg 细胞分化并影响乳腺癌的免疫逃逸。
Int J Biol Macromol. 2018 Oct 15;118(Pt A):24-30. doi: 10.1016/j.ijbiomac.2018.06.033. Epub 2018 Jun 8.