Heilongjiang Provinical Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3093-3099. doi: 10.1099/ijsem.0.003597. Epub 2019 Jul 23.
A novel bacterial strain, designated NEAU-SA2, was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-flagellated and rod-coccus-shaped. The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus and was closely related to DSM 24664 (98.89 %) and CCTCC AB 2017271 (98.41 %), which was further confirmed by multilocus sequence analysis. The major cellular fatty acids were -C, -C and C; MK-9(H) was the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose. The genomic G+C content of strain NEAU-SA2 was 67.04 mol%. The average nucleotide identity values between NEAU-SA2 and DSM 24664 and CCTCC AB 2017271 were 88.57-90.94 %. The digital DNA-DNA hybridisation values between strain NEAU-SA2 and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa. Therefore, strain NEAU-SA2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-SA2 (=DSM 106718=CCTCC AB 2017272).
一株新的细菌菌株，命名为 NEAU-SA2，从中国湖南省张家界市采集的森林土壤中分离出来，并通过多相方法进行了特征描述。该细胞为需氧、革兰氏阳性、无鞭毛和杆状-球菌形状。该菌株在 28°C、pH7.0 和 0%NaCl（w/v）下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，该生物应属于 属，与 DSM 24664（98.89%）和 CCTCC AB 2017271（98.41%）最为密切相关，这进一步通过多位点序列分析得到证实。主要的细胞脂肪酸为 -C、-C 和 C；MK-9(H) 是主要的呼吸醌。极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未鉴定的糖脂。肽聚糖类型为 A3α，细胞壁糖为葡萄糖和半乳糖。菌株 NEAU-SA2 的基因组 G+C 含量为 67.04mol%。NEAU-SA2 与 DSM 24664 和 CCTCC AB 2017271 的平均核苷酸同一性值分别为 88.57-90.94%。NEAU-SA2 与最密切相关的种之间的数字 DNA-DNA 杂交值分别为 37.00%和 41.10%，这再次表明它们属于不同的分类单元。因此，菌株 NEAU-SA2 代表了 属的一个新种，建议使用 sp. nov. 来命名。其模式菌株为 NEAU-SA2（=DSM 106718=CCTCC AB 2017272）。
