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从 中鉴定和量化一种具有植物毒性的代谢物。

Identification and Quantification of a Phytotoxic Metabolite from .

机构信息

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, 97205 Mérida, Yucatán, Mexico.

UPRES EA921SONAS, SFR 4207 QUASAV, Université d'Angers, 49045 Angers, France.

出版信息

Molecules. 2020 Sep 2;25(17):4003. doi: 10.3390/molecules25174003.

DOI:10.3390/molecules25174003
PMID:32887350
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7504779/
Abstract

is the causal agent of leaf blight (ALB) in carrot () crops around the world. However, to date, has received limited attention in its production of phytotoxic metabolites. In this investigation, the bioassay-guided isolation of the extract from liquid cultures of resulted in the isolation of two metabolites identified as -acetylorcinol () and -hydroxybenzoic acid (), based on their spectroscopic data and results from chemical correlation reactions. Testing of both metabolites in different assays showed an important phytotoxic activity for -hydroxybenzoic acid () when tested in the leaf-spot assay on parsley (), in the leaf infiltration assay on tobacco () and marigold (), and in the immersion assay on parsley and parsnip () leaves. Quantification of the two metabolites in the crude extract of kept at different times showed that -hydroxybenzoic acid () is one of the first metabolites to be synthesized by the pathogen, suggesting that this salicylic acid derivative could play an important role in the pathogenicity of the fungus.

摘要

是世界范围内胡萝卜（）作物叶枯病（ALB）的病原体。然而，迄今为止，在其产生的植物毒性代谢物方面，受到的关注有限。在本研究中，通过对液体培养物提取物进行生物测定指导分离，从 中分离出两种代谢物，根据其光谱数据和化学相关反应的结果，鉴定为 -乙酰邻苯二酚（）和 -羟基苯甲酸（）。对两种代谢物进行的不同测定表明，-羟基苯甲酸（）在欧芹（）叶斑测定、烟草（）和万寿菊（）叶片的叶内渗透测定以及欧芹和欧洲防风草（）叶片的浸泡测定中均具有重要的植物毒性活性。对不同时间保存的 粗提物中两种代谢物的定量表明，-羟基苯甲酸（）是病原体最早合成的代谢物之一，表明这种水杨酸衍生物可能在真菌的致病性中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/1584b693f5cb/molecules-25-04003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/8617573e3d3e/molecules-25-04003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/ed9999cfc262/molecules-25-04003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/d5c858dc0991/molecules-25-04003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/17578ad5be23/molecules-25-04003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/1584b693f5cb/molecules-25-04003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/8617573e3d3e/molecules-25-04003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/ed9999cfc262/molecules-25-04003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/d5c858dc0991/molecules-25-04003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/17578ad5be23/molecules-25-04003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8259/7504779/1584b693f5cb/molecules-25-04003-g005.jpg

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