Department of Bioengineering, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey.
Department of Chemistry, Kütahya Dumlupınar University, 43100, Kütahya, Turkey.
Mikrochim Acta. 2020 Sep 4;187(10):549. doi: 10.1007/s00604-020-04526-x.
Cancer is one of the most common and important diseases with a high mortality rate. Breast cancer is among the three most common types of cancer in women, and the mortality rate has reached 0.024% in some countries. For early-stage preclinical diagnosis of breast cancer, sensitive and reliable tools are needed. Today, there are many types of biomarkers that have been identified for cancer diagnosis. A wide variety of detection strategies have also been developed for the detection of these biomarkers from serum or other body fluids at physiological concentrations. Aptamers are single-stranded DNA or RNA oligonucleotides and promising in the production of more sensitive and reliable biosensor platforms in combination with a wide range of nanomaterials. Conformational changes triggered by the target analyte have been successfully applied in fluorometric, colorimetric, plasmonic, and electrochemical-based detection strategies. This review article presents aptasensor approaches used in the detection of vascular endothelial growth factor (VEGF), human epidermal growth factor receptor 2 (HER2), and mucin-1 glycoprotein (MUC1) biomarkers, which are frequently studied in the diagnosis of breast cancer. The focus of this review article is on developments of the last decade for detecting these biomarkers using various sensitivity enhancement techniques and nanomaterials.
癌症是一种常见且重要的疾病，死亡率较高。乳腺癌是女性中最常见的三种癌症之一，在一些国家的死亡率已达到 0.024%。对于乳腺癌的早期临床前诊断，需要敏感且可靠的工具。如今，已经确定了许多用于癌症诊断的生物标志物类型。为了在生理浓度下从血清或其他体液中检测这些生物标志物，已经开发了各种检测策略。适体是单链 DNA 或 RNA 寡核苷酸，与各种纳米材料结合使用，在生产更敏感和可靠的生物传感器平台方面具有广阔的前景。靶分析物触发的构象变化已成功应用于荧光、比色、等离子体和基于电化学的检测策略中。本文综述了用于检测血管内皮生长因子 (VEGF)、人表皮生长因子受体 2 (HER2) 和粘蛋白 1 糖蛋白 (MUC1) 等生物标志物的适体传感器方法，这些生物标志物在乳腺癌诊断中经常被研究。本文的重点是过去十年使用各种灵敏度增强技术和纳米材料检测这些生物标志物的发展。
